Impact of maturational status on the ability of osteoblasts to enhance the hematopoietic function of stem and progenitor cells

Ying Hua Cheng, Brahmananda R. Chitteti, Drew A. Streicher, Joseph A. Morgan, Sonia Rodriguez-Rodriguez, Nadia Carlesso, Edward Srour, Melissa Kacena

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Osteoblasts (OBs) exert a prominent regulatory effect on hematopoietic stem cells (HSCs). We evaluated the difference in hematopoietic expansion and function in response to co-culture with OBs at various stages of development. Murine calvarial OBs were seeded directly (fresh) or cultured for 1, 2, or 3 weeks prior to seeding with 1000 Lin-Sca1 +cKit+ (LSK) cells for 1 week. Significant increases in the following hematopoietic parameters were detected when comparing co-cultures of fresh OBs to co-cultures containing OBs cultured for 1, 2, or 3 weeks: total hematopoietic cell number (up to a 3.4-fold increase), total colony forming unit (CFU) number in LSK progeny (up to an 18.1-fold increase), and percentage of Lin-Sca1+ cells (up to a 31.8-fold increase). Importantly, these studies were corroborated by in vivo reconstitution studies in which LSK cells maintained in fresh OB co-cultures supported a significantly higher level of chimerism than cells maintained in co-cultures containing 3-week OBs. Characterization of OBs cultured for 1, 2, or 3 weeks with real-time PCR and functional mineralization assays showed that OB maturation increased with culture duration but was not affected by the presence of LSK cells in culture. Linear regression analyses of multiple parameters measured in these studies show that fresh, most likely more immature OBs better promote hematopoietic expansion and function than cultured, presumably more mature OBs and suggest that the hematopoiesis-enhancing activity is mediated by cells present in fresh OB cultures de novo.

Original languageEnglish
Pages (from-to)1111-1121
Number of pages11
JournalJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Volume26
Issue number5
DOIs
StatePublished - May 2011

Fingerprint

Hematopoietic Stem Cells
Osteoblasts
Coculture Techniques
Chimerism
Hematopoiesis
Real-Time Polymerase Chain Reaction
Linear Models
Stem Cells
Cell Culture Techniques
Cell Count
Regression Analysis

Keywords

  • CALCIUM DEPOSITION
  • HEMATOPOIETIC NICHE
  • HEMATOPOIETIC STEM CELLS
  • MOUSE
  • OSTEOBLASTS

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Impact of maturational status on the ability of osteoblasts to enhance the hematopoietic function of stem and progenitor cells. / Cheng, Ying Hua; Chitteti, Brahmananda R.; Streicher, Drew A.; Morgan, Joseph A.; Rodriguez-Rodriguez, Sonia; Carlesso, Nadia; Srour, Edward; Kacena, Melissa.

In: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 26, No. 5, 05.2011, p. 1111-1121.

Research output: Contribution to journalArticle

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AU - Srour, Edward

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