Impact of Pregnancy History and 17-Hydroxyprogesterone Caproate on Cervical Cytokines and Matrix Metalloproteinases

Steve N. Caritis, Gary Hankins, Mary Hebert, David Haas, Mahmoud Ahmed, Hyagriv Simhan, Laura Haneline, John Harris, Justine Chang, Alyssa Stephenson Famy, Patrick Yorio, Zhaoxia Ren, Mary E. D'alton, Raman Venkataramanan

Research output: Contribution to journalArticle

Abstract

Objective The objective of this study was to evaluate the impact of pregnancy history and 17-hydroxyprogesterone caproate (17-OHPC) treatment on cervical fluid cytokines and matrix metalloproteinases (MMPs). Study Design Cervical fluid was obtained between 16 0/7 and 24 6/7 weeks from women with only prior term births (controls, n = 26), women with one or more prior spontaneous preterm births (SPTBs) choosing to receive 17-OHPC (17-OHPC, n = 24), or to not receive 17-OHPC (refusers, n = 12). Cervical fluid collections were repeated 2, 4, and 8 weeks after the first sample and concentrations of MMPs and cytokines were measured by multiplex immune assay. Results Among women whose earliest prior delivery occurred between 16 and 23 weeks, cervical fluid concentration of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α at baseline were significantly elevated when compared with cervical cytokines of women whose earliest delivery occurred between 32 and 36 weeks (relative risk ratio was 3.37 for IL-6 [95% confidence interval, CI, 1.08–10.53, p < 0.05], 2.81 for IL-10 [95% CI, 1.39–5.70, p < 0.05], and 6.34 for TNF-α [95% CI, 2.19–18.68, p < 0.001]). Treatment with 17-OHPC had no significant impact on these cytokines. Conclusion The cervical fluid of women with a history of an early prior SPTB is characterized by inflammation that is unaffected by 17-OHPC.

Original languageEnglish (US)
JournalAmerican Journal of Perinatology
DOIs
StateAccepted/In press - Nov 15 2017

Fingerprint

17-alpha-Hydroxyprogesterone
Reproductive History
Matrix Metalloproteinases
Cytokines
Premature Birth
Interleukin-10
Interleukin-6
Tumor Necrosis Factor-alpha
Term Birth
Contraception
Odds Ratio
17-alpha-hydroxy-progesterone caproate
Confidence Intervals
Inflammation
Therapeutics

Keywords

  • 17-hydroxyprogesterone caproate
  • cervicovaginal
  • cytokines
  • inflammatory milieu
  • matrix metalloproteinases

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Cite this

Impact of Pregnancy History and 17-Hydroxyprogesterone Caproate on Cervical Cytokines and Matrix Metalloproteinases. / Caritis, Steve N.; Hankins, Gary; Hebert, Mary; Haas, David; Ahmed, Mahmoud; Simhan, Hyagriv; Haneline, Laura; Harris, John; Chang, Justine; Famy, Alyssa Stephenson; Yorio, Patrick; Ren, Zhaoxia; D'alton, Mary E.; Venkataramanan, Raman.

In: American Journal of Perinatology, 15.11.2017.

Research output: Contribution to journalArticle

Caritis, SN, Hankins, G, Hebert, M, Haas, D, Ahmed, M, Simhan, H, Haneline, L, Harris, J, Chang, J, Famy, AS, Yorio, P, Ren, Z, D'alton, ME & Venkataramanan, R 2017, 'Impact of Pregnancy History and 17-Hydroxyprogesterone Caproate on Cervical Cytokines and Matrix Metalloproteinases', American Journal of Perinatology. https://doi.org/10.1055/s-0037-1608631
Caritis, Steve N. ; Hankins, Gary ; Hebert, Mary ; Haas, David ; Ahmed, Mahmoud ; Simhan, Hyagriv ; Haneline, Laura ; Harris, John ; Chang, Justine ; Famy, Alyssa Stephenson ; Yorio, Patrick ; Ren, Zhaoxia ; D'alton, Mary E. ; Venkataramanan, Raman. / Impact of Pregnancy History and 17-Hydroxyprogesterone Caproate on Cervical Cytokines and Matrix Metalloproteinases. In: American Journal of Perinatology. 2017.
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abstract = "Objective The objective of this study was to evaluate the impact of pregnancy history and 17-hydroxyprogesterone caproate (17-OHPC) treatment on cervical fluid cytokines and matrix metalloproteinases (MMPs). Study Design Cervical fluid was obtained between 16 0/7 and 24 6/7 weeks from women with only prior term births (controls, n = 26), women with one or more prior spontaneous preterm births (SPTBs) choosing to receive 17-OHPC (17-OHPC, n = 24), or to not receive 17-OHPC (refusers, n = 12). Cervical fluid collections were repeated 2, 4, and 8 weeks after the first sample and concentrations of MMPs and cytokines were measured by multiplex immune assay. Results Among women whose earliest prior delivery occurred between 16 and 23 weeks, cervical fluid concentration of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α at baseline were significantly elevated when compared with cervical cytokines of women whose earliest delivery occurred between 32 and 36 weeks (relative risk ratio was 3.37 for IL-6 [95{\%} confidence interval, CI, 1.08–10.53, p < 0.05], 2.81 for IL-10 [95{\%} CI, 1.39–5.70, p < 0.05], and 6.34 for TNF-α [95{\%} CI, 2.19–18.68, p < 0.001]). Treatment with 17-OHPC had no significant impact on these cytokines. Conclusion The cervical fluid of women with a history of an early prior SPTB is characterized by inflammation that is unaffected by 17-OHPC.",
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T1 - Impact of Pregnancy History and 17-Hydroxyprogesterone Caproate on Cervical Cytokines and Matrix Metalloproteinases

AU - Caritis, Steve N.

AU - Hankins, Gary

AU - Hebert, Mary

AU - Haas, David

AU - Ahmed, Mahmoud

AU - Simhan, Hyagriv

AU - Haneline, Laura

AU - Harris, John

AU - Chang, Justine

AU - Famy, Alyssa Stephenson

AU - Yorio, Patrick

AU - Ren, Zhaoxia

AU - D'alton, Mary E.

AU - Venkataramanan, Raman

PY - 2017/11/15

Y1 - 2017/11/15

N2 - Objective The objective of this study was to evaluate the impact of pregnancy history and 17-hydroxyprogesterone caproate (17-OHPC) treatment on cervical fluid cytokines and matrix metalloproteinases (MMPs). Study Design Cervical fluid was obtained between 16 0/7 and 24 6/7 weeks from women with only prior term births (controls, n = 26), women with one or more prior spontaneous preterm births (SPTBs) choosing to receive 17-OHPC (17-OHPC, n = 24), or to not receive 17-OHPC (refusers, n = 12). Cervical fluid collections were repeated 2, 4, and 8 weeks after the first sample and concentrations of MMPs and cytokines were measured by multiplex immune assay. Results Among women whose earliest prior delivery occurred between 16 and 23 weeks, cervical fluid concentration of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α at baseline were significantly elevated when compared with cervical cytokines of women whose earliest delivery occurred between 32 and 36 weeks (relative risk ratio was 3.37 for IL-6 [95% confidence interval, CI, 1.08–10.53, p < 0.05], 2.81 for IL-10 [95% CI, 1.39–5.70, p < 0.05], and 6.34 for TNF-α [95% CI, 2.19–18.68, p < 0.001]). Treatment with 17-OHPC had no significant impact on these cytokines. Conclusion The cervical fluid of women with a history of an early prior SPTB is characterized by inflammation that is unaffected by 17-OHPC.

AB - Objective The objective of this study was to evaluate the impact of pregnancy history and 17-hydroxyprogesterone caproate (17-OHPC) treatment on cervical fluid cytokines and matrix metalloproteinases (MMPs). Study Design Cervical fluid was obtained between 16 0/7 and 24 6/7 weeks from women with only prior term births (controls, n = 26), women with one or more prior spontaneous preterm births (SPTBs) choosing to receive 17-OHPC (17-OHPC, n = 24), or to not receive 17-OHPC (refusers, n = 12). Cervical fluid collections were repeated 2, 4, and 8 weeks after the first sample and concentrations of MMPs and cytokines were measured by multiplex immune assay. Results Among women whose earliest prior delivery occurred between 16 and 23 weeks, cervical fluid concentration of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α at baseline were significantly elevated when compared with cervical cytokines of women whose earliest delivery occurred between 32 and 36 weeks (relative risk ratio was 3.37 for IL-6 [95% confidence interval, CI, 1.08–10.53, p < 0.05], 2.81 for IL-10 [95% CI, 1.39–5.70, p < 0.05], and 6.34 for TNF-α [95% CI, 2.19–18.68, p < 0.001]). Treatment with 17-OHPC had no significant impact on these cytokines. Conclusion The cervical fluid of women with a history of an early prior SPTB is characterized by inflammation that is unaffected by 17-OHPC.

KW - 17-hydroxyprogesterone caproate

KW - cervicovaginal

KW - cytokines

KW - inflammatory milieu

KW - matrix metalloproteinases

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U2 - 10.1055/s-0037-1608631

DO - 10.1055/s-0037-1608631

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JO - American Journal of Perinatology

JF - American Journal of Perinatology

SN - 0735-1631

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