Impaired agonist-stimulated intracellular free calcium (Ca i) responses in aortic endothelial cells freshly dispersed from endotoxemic guinea pigs

J. J. Jones, Michael Sturek, O. Zhong, H. R. Adams, J. L. Parker

Research output: Contribution to journalArticle

Abstract

We recently reported evidence for impairment of endothelium-dependent relaxation (EDR) and decreased agonist-stimulated endothelial synthesis/release of nitric oxide (NO) using in vitro aortic and coronary preparations isolated from endotoxemic guinea pigs. We tested the hypothesis that impaired EUR involves dysfunctional endothelial Ca i regulation by using fura-2 microfluorometry to compare agonist-stimulated Ca i responses of endothelial cells (EC) 16 h after intmperiloneal injection of bacterial endotoxin (LPS; 4 mg/kg] or saline (CON). Basal Ca i (340/380 nm fluorescence ratio. R) was not different in CON and LPS EC (1.1±.03 and 1.1±03 respectively). Exposure of EC to ADP (10 μM), in the presence of extracellular Ca 2+ (2mM) produced hiphasic increases in Ca i that were markedly decreased (Pi responses to ACh. Moreover. ACh (but not ADP) Ca i responses rapidly decreased with time (within 2 h) after dispersion. In conclusion, our data demonstrate impaired agonist -stimulated endothelial Ca i mobilization; the dependence of NO synthesis on Ca i suggests that Ca, defects may contribute to LPS-induced impaired EDR.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Endothelial cells
guinea pigs
endothelial cells
agonists
Guinea Pigs
Endothelial Cells
Calcium
calcium
endothelium
Adenosine Diphosphate
Endothelium
nitric oxide
Nitric Oxide
Cytophotometry
synthesis
Fura-2
endotoxins
Endotoxins
Fluorescence
fluorescence

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Impaired agonist-stimulated intracellular free calcium (Ca i) responses in aortic endothelial cells freshly dispersed from endotoxemic guinea pigs. / Jones, J. J.; Sturek, Michael; Zhong, O.; Adams, H. R.; Parker, J. L.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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AU - Parker, J. L.

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