Impaired Collateral Artery Development in Spontaneously Hypertensive Rats

Jay L. Tuttle, Bridget M. Sanders, Harold M. Burkhart, Steven W. Fath, Kimberly A. Kerr, William C. Watson, B. Herring, Michael Dalsing, Joseph L. Unthank

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Objective: To determine whether collateral artery development is impaired in spontaneously hypertensive (SHR) relative to normotensive (WKY) rats. Methods: Sequential mesenteric arteries were ligated to create a collateral pathway responsible for the perfusion of approximately 50 first-order arterioles. Collateral development was assessed by measurement of in vivo arterial diameter before and 1 week after ligation. Histological arid morphometric measurements were made from cross-sectional preparations of these arteries to evaluate intimal and medial cell numbers and medial area. eNOS expression was evaluated with Western blotting. Results: One week after arterial ligation, collateral diameter was increased more in WKY than SHR both absolutely (137 ± 9.1 versus 99 ± 8.6 μm) and relative to same-animal controls (38 ± 5.5% versus 13 ± 7.1%). At the time of model creation, blood flow was elevated to comparable levels in both WKY and SHR, and wall shear rate in the SHR collateral was greater than both the SHR control and WKY collateral arteries. Endothelial cell number in arterial cross-section was increased in collaterals by 80% in WKY and only 22% in the SHR. eNOS expression was increased in the WKY (128%) but not in the SHR collateral. Conclusions: For equivalent arterial occlusion, the data demonstrate that collateral development is suppressed in the SHR as indicated by luminal expansion. This impairment of luminal expansion is associated with a decreased endothelial proliferation and the lack of an increase in eNOS expression.

Original languageEnglish
Pages (from-to)343-351
Number of pages9
JournalMicrocirculation
Volume9
Issue number5
DOIs
StatePublished - 2002

Fingerprint

Inbred SHR Rats
Arteries
Ligation
Cell Count
Tunica Intima
Mesenteric Arteries
Inbred WKY Rats
Arterioles
Endothelial Cells
Perfusion
Western Blotting

Keywords

  • Collateral arteries
  • Collateral development
  • Flow-mediated remodeling
  • Mesenteric artery
  • Spontaneously hypertensive rat

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Cardiology and Cardiovascular Medicine

Cite this

Tuttle, J. L., Sanders, B. M., Burkhart, H. M., Fath, S. W., Kerr, K. A., Watson, W. C., ... Unthank, J. L. (2002). Impaired Collateral Artery Development in Spontaneously Hypertensive Rats. Microcirculation, 9(5), 343-351. https://doi.org/10.1038/sj.mn.7800151

Impaired Collateral Artery Development in Spontaneously Hypertensive Rats. / Tuttle, Jay L.; Sanders, Bridget M.; Burkhart, Harold M.; Fath, Steven W.; Kerr, Kimberly A.; Watson, William C.; Herring, B.; Dalsing, Michael; Unthank, Joseph L.

In: Microcirculation, Vol. 9, No. 5, 2002, p. 343-351.

Research output: Contribution to journalArticle

Tuttle, JL, Sanders, BM, Burkhart, HM, Fath, SW, Kerr, KA, Watson, WC, Herring, B, Dalsing, M & Unthank, JL 2002, 'Impaired Collateral Artery Development in Spontaneously Hypertensive Rats', Microcirculation, vol. 9, no. 5, pp. 343-351. https://doi.org/10.1038/sj.mn.7800151
Tuttle JL, Sanders BM, Burkhart HM, Fath SW, Kerr KA, Watson WC et al. Impaired Collateral Artery Development in Spontaneously Hypertensive Rats. Microcirculation. 2002;9(5):343-351. https://doi.org/10.1038/sj.mn.7800151
Tuttle, Jay L. ; Sanders, Bridget M. ; Burkhart, Harold M. ; Fath, Steven W. ; Kerr, Kimberly A. ; Watson, William C. ; Herring, B. ; Dalsing, Michael ; Unthank, Joseph L. / Impaired Collateral Artery Development in Spontaneously Hypertensive Rats. In: Microcirculation. 2002 ; Vol. 9, No. 5. pp. 343-351.
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AU - Kerr, Kimberly A.

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AU - Unthank, Joseph L.

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