Impaired collateral development in mature rats

Jay L. Tuttle, Tara L. Hahn, Bridget M. Sanders, Frank A. Witzmann, Steven J. Miller, Michael C. Dalsing, Joseph L. Unthank

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

The effect of maturation on collateral development of resistance arteries was investigated. Three to four sequential mesenteric arteries were ligated to create collateral pathways in anesthetized young (∼200 g) and mature (∼600 g) rats. Blood flow was similarly elevated in collaterals of young and mature animals. In vivo inner arterial diameter was increased only within young collaterals (33 ± 7%, P < 0.001). Increases in number of intimal nuclei (57 ± 10% vs. 52 ± 14%) and cross-sectional medial area (33 ± 13% vs. 38 ± 5%) were similar between young and mature collaterals. Relative to the same animal controls, collateral endothelial nitric oxide synthase mRNA was increased as much in mature as in young rats. Proteomic analysis revealed significant differences in protein expression with maturation between control arteries as well as flow-loaded collateral vessels. The results indicate that, whereas intimal and medial remodeling events were similar in collaterals of young and mature rats, luminal expansion occurred only in young rats. Alteration in arterial protein expression with maturation and altered responses to stimuli for collateral development may contribute to this impairment.

Original languageEnglish (US)
Pages (from-to)H146-H155
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume283
Issue number1 52-1
StatePublished - Jul 10 2002

Keywords

  • Arterial remodeling
  • eNOS
  • Luminal expansion
  • Proteomic analysis
  • Resistance artery

ASJC Scopus subject areas

  • Physiology

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