Impaired glucose tolerance and predisposition to the fasted state in liver glycogen synthase knock-out mice

Jose M. Irimia, Catalina M. Meyer, Caron L. Peper, Lanmin Zhai, Cheryl B. Bock, Stephen F. Previs, Owen P. McGuinness, Anna De Paoli-Roach, Peter Roach

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Conversion to glycogen is a major fate of ingested glucose in the body. A rate-limiting enzyme in the synthesis of glycogen is glycogen synthase encoded by two genes, GYS1, expressed in muscle and other tissues, and GYS2, primarily expressed in liver (liver glycogen synthase). Defects in GYS2 cause the inherited monogenic disease glycogen storage disease 0. We have generated mice with a liver-specific disruption of the Gys2 gene (liver glycogen synthase knock-out (LGSKO) mice), using Lox-P/Cre technology. Conditional mice carrying floxed Gys2 were crossed with mice expressing Cre recombinase under the albumin promoter. The resulting LGSKO mice are viable, develop liver glycogen synthase deficiency, and have a 95% reduction in fed liver glycogen content. They have mild hypoglycemia but dispose glucose less well in a glucose tolerance test. Fed, LGSKO mice also have a reduced capacity for exhaustive exercise compared with mice carrying floxed alleles, but the difference disappears after an overnight fast. Upon fasting, LGSKO mice reach within 4 h decreased blood glucose levels attained by control floxed mice only after 24 h of food deprivation. The LGSKO mice maintain this low blood glucose for at least 24 h. Basal gluconeogenesis is increased in LGSKO mice, and insulin suppression of endogenous glucose production is impaired as assessed by euglycemic- hyperinsulinemic clamp. This observation correlates with an increase in the liver gluconeogenic enzyme phosphoenolpyruvate carboxykinase expression and activity. This mouse model mimics the pathophysiology of glycogen storage disease 0 patients and highlights the importance of liver glycogen stores in whole body glucose homeostasis.

Original languageEnglish
Pages (from-to)12851-12861
Number of pages11
JournalJournal of Biological Chemistry
Volume285
Issue number17
DOIs
StatePublished - Apr 23 2010

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Glycogen Synthase
Liver Glycogen
Glucose Intolerance
Knockout Mice
Glucose
Glycogen
Glycogen Storage Disease
Liver
Blood Glucose
Genes
Food Deprivation
Phosphoenolpyruvate
Glucose Clamp Technique
Gluconeogenesis
Enzymes
Glucose Tolerance Test
Clamping devices
Hypoglycemia
Albumins
Fasting

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Impaired glucose tolerance and predisposition to the fasted state in liver glycogen synthase knock-out mice. / Irimia, Jose M.; Meyer, Catalina M.; Peper, Caron L.; Zhai, Lanmin; Bock, Cheryl B.; Previs, Stephen F.; McGuinness, Owen P.; De Paoli-Roach, Anna; Roach, Peter.

In: Journal of Biological Chemistry, Vol. 285, No. 17, 23.04.2010, p. 12851-12861.

Research output: Contribution to journalArticle

Irimia, Jose M. ; Meyer, Catalina M. ; Peper, Caron L. ; Zhai, Lanmin ; Bock, Cheryl B. ; Previs, Stephen F. ; McGuinness, Owen P. ; De Paoli-Roach, Anna ; Roach, Peter. / Impaired glucose tolerance and predisposition to the fasted state in liver glycogen synthase knock-out mice. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 17. pp. 12851-12861.
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