Impaired IgG production in the lungs of HIV-infected individuals

Homer Twigg, Blake A. Spain, Diaa M. Soliman, Linda K. Bowen, Kathleen M. Heidler, David S. Wilkes

Research output: Contribution to journalArticle

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Abstract

Human immunodeficiency virus (HIV)-infected individuals are at risk for pulmonary infections with encapsulated bacterial pathogens. This could reflect impaired production of opsonizing antibodies in the lower respiratory tract. We examined antibody production in the alveolar space by measuring immunoglobulin concentrations in bronchoalveolar lavage (BAL) of HIV-infected patients and normal volunteers and by assessing the ability of alveolar macrophages (AM) to induce immunoglobulin production in normal peripheral blood mononuclear cells (PBMC). BAL from HIV-infected patients contained significantly less IgG than normal BAL. IgA and IgM concentrations were similar in both groups. Normal AM supported IgG and IgA production in PBMC. While HIV AM could induce IgA production in PBMC, in no instance did they induce IgG secretion. HIV AM produced significantly more transforming growth factor-β (TGF-β), a factor known to suppress IgG production, than normal AM. Finally, TGF-β antibodies blocked the inhibitory effect of HIV AM on normal IgG secretion without affecting IgA secretion. These findings demonstrate impaired production of opsonizing IgG in the alveolar space of HIV-infected subjects and implicate excess TGF-β production by AM as the cause of this impairment.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalCellular Immunology
Volume170
Issue number1
DOIs
StatePublished - May 25 1996

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Alveolar Macrophages
Immunoglobulin G
HIV
Lung
Immunoglobulin A
Transforming Growth Factors
Bronchoalveolar Lavage
Blood Cells
Antibody Formation
Immunoglobulins
Respiratory System
Immunoglobulin M
Healthy Volunteers
Antibodies
Infection

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Twigg, H., Spain, B. A., Soliman, D. M., Bowen, L. K., Heidler, K. M., & Wilkes, D. S. (1996). Impaired IgG production in the lungs of HIV-infected individuals. Cellular Immunology, 170(1), 127-133. https://doi.org/10.1006/cimm.1996.0142

Impaired IgG production in the lungs of HIV-infected individuals. / Twigg, Homer; Spain, Blake A.; Soliman, Diaa M.; Bowen, Linda K.; Heidler, Kathleen M.; Wilkes, David S.

In: Cellular Immunology, Vol. 170, No. 1, 25.05.1996, p. 127-133.

Research output: Contribution to journalArticle

Twigg, H, Spain, BA, Soliman, DM, Bowen, LK, Heidler, KM & Wilkes, DS 1996, 'Impaired IgG production in the lungs of HIV-infected individuals', Cellular Immunology, vol. 170, no. 1, pp. 127-133. https://doi.org/10.1006/cimm.1996.0142
Twigg H, Spain BA, Soliman DM, Bowen LK, Heidler KM, Wilkes DS. Impaired IgG production in the lungs of HIV-infected individuals. Cellular Immunology. 1996 May 25;170(1):127-133. https://doi.org/10.1006/cimm.1996.0142
Twigg, Homer ; Spain, Blake A. ; Soliman, Diaa M. ; Bowen, Linda K. ; Heidler, Kathleen M. ; Wilkes, David S. / Impaired IgG production in the lungs of HIV-infected individuals. In: Cellular Immunology. 1996 ; Vol. 170, No. 1. pp. 127-133.
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