Impaired Store-Operated Calcium Entry and STIM1 Loss Lead to Reduced Insulin Secretion and Increased Endoplasmic Reticulum Stress in the Diabetic β-Cell

Tatsuyoshi Kono, Xin Tong, Carmella Evans-Molina, Tatsuyoshi Kono, Hitoshi Iida, Chih Chun Lee, Paul Sohn, Patrick Gilon, Michael W. Roe, Carmella Evans-Molina

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Store-operated Ca2+ entry (SOCE) is a dynamic process that leads to refilling of endoplasmic reticulum (ER) Ca2+ stores through reversible gating of plasma membrane Ca2+ channels by the ER Ca2+ sensor Stromal Interaction Molecule 1 (STIM1). Pathogenic reductions in β-cell ER Ca2+ have been observed in diabetes. However, a role for impaired SOCE in this phenotype has not been tested. We measured the expression of SOCE molecular components in human and rodent models of diabetes and found a specific reduction in STIM1 mRNA and protein levels in human islets from donors with type 2 diabetes (T2D), islets from hyperglycemic streptozotocin-treated mice, and INS-1 cells (rat insulinoma cells) treated with proinflammatory cytokines and palmitate. Pharmacologic SOCE inhibitors led to impaired islet Ca2+ oscillations and insulin secretion, and these effects were phenocopied by β-cell STIM1 deletion. STIM1 deletion also led to reduced ER Ca2+ storage and increased ER stress, whereas STIM1 gain of function rescued β-cell survival under proinflammatory conditions and improved insulin secretion in human islets from donors with T2D. Taken together, these data suggest that the loss of STIM1 and impaired SOCE contribute to ER Ca2+ dyshomeostasis under diabetic conditions, whereas efforts to restore SOCE-mediated Ca2+ transients may have the potential to improve β-cell health and function.

Original languageEnglish (US)
Pages (from-to)2293-2304
Number of pages12
JournalDiabetes
Volume67
Issue number11
DOIs
StatePublished - Nov 1 2018

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Endoplasmic Reticulum Stress
Endoplasmic Reticulum
Insulin
Calcium
Type 2 Diabetes Mellitus
Insulinoma
Palmitates
Streptozocin
Ion Channels
Cell Communication
Stromal Interaction Molecule 1
Rodentia
Cell Survival
Cell Membrane
Cytokines
Phenotype
Messenger RNA
Health
Proteins

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Impaired Store-Operated Calcium Entry and STIM1 Loss Lead to Reduced Insulin Secretion and Increased Endoplasmic Reticulum Stress in the Diabetic β-Cell. / Kono, Tatsuyoshi; Tong, Xin; Evans-Molina, Carmella; Kono, Tatsuyoshi; Iida, Hitoshi; Lee, Chih Chun; Sohn, Paul; Gilon, Patrick; Roe, Michael W.; Evans-Molina, Carmella.

In: Diabetes, Vol. 67, No. 11, 01.11.2018, p. 2293-2304.

Research output: Contribution to journalArticle

Kono, Tatsuyoshi ; Tong, Xin ; Evans-Molina, Carmella ; Kono, Tatsuyoshi ; Iida, Hitoshi ; Lee, Chih Chun ; Sohn, Paul ; Gilon, Patrick ; Roe, Michael W. ; Evans-Molina, Carmella. / Impaired Store-Operated Calcium Entry and STIM1 Loss Lead to Reduced Insulin Secretion and Increased Endoplasmic Reticulum Stress in the Diabetic β-Cell. In: Diabetes. 2018 ; Vol. 67, No. 11. pp. 2293-2304.
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