Impaired tubular uptake explains albuminuria in early diabetic nephropathy

Leileata M. Russo, Ruben M. Sandoval, Silvia Campos-Bilderback, Bruce Molitoris, Wayne D. Comper, Dennis Brown

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Understanding the pathogenesis of albuminuria in diabetic nephropathy is important to improve methods for early diagnosis and treatment. In this study, we addressed whether albuminuria in diabetes results from altered glomerular filtration and/or altered processing of filtered albumin by the proximal tubule. Type 1 diabetic Munich Wistar rats developed albuminuria after 12 wk of diabetes. Intravital two-photon microscopy revealed similar glomerular permeability in the diabetic and control animals, assessed using both albumin-Alexa568 and 69-kD FITC-dextran; however, diabetic animals demonstrated significantly less filtered fluorescent albumin in renal proximal tubule (PT) cells compared with control animals. We also observed increased albumin-derived urinary peptide excretion in diabetic animals, and hyperglycemia modulated this peptideuria. In conclusion, in the early stages of diabetic nephropathy, the PT plays a major role in the development of albuminuria, which may be preceded by peptideuria.

Original languageEnglish
Pages (from-to)489-494
Number of pages6
JournalJournal of the American Society of Nephrology
Volume20
Issue number3
DOIs
StatePublished - Mar 2009

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Albuminuria
Diabetic Nephropathies
Albumins
Proximal Kidney Tubule
Photons
Hyperglycemia
Wistar Rats
Early Diagnosis
Microscopy
Permeability
Peptides

ASJC Scopus subject areas

  • Nephrology

Cite this

Impaired tubular uptake explains albuminuria in early diabetic nephropathy. / Russo, Leileata M.; Sandoval, Ruben M.; Campos-Bilderback, Silvia; Molitoris, Bruce; Comper, Wayne D.; Brown, Dennis.

In: Journal of the American Society of Nephrology, Vol. 20, No. 3, 03.2009, p. 489-494.

Research output: Contribution to journalArticle

Russo, Leileata M. ; Sandoval, Ruben M. ; Campos-Bilderback, Silvia ; Molitoris, Bruce ; Comper, Wayne D. ; Brown, Dennis. / Impaired tubular uptake explains albuminuria in early diabetic nephropathy. In: Journal of the American Society of Nephrology. 2009 ; Vol. 20, No. 3. pp. 489-494.
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