Importance of N-linked glycosylation in the functional expression of murine CD1d1

Venkataraman Sriram, Claire A. Willard, Jianyun Liu, Randy R. Brutkiewicz

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The mouse CD1d1 glycoprotein is specialized in presenting lipid antigens to a novel class of T cells called natural killer T (NKT) cells. CD1d1 is predicted to contain five potential N-linked glycosylation sites (asparagine residues at positions 25, 38, 60, 128, and 183). Glycosylation has been shown to invariably affect the molecular and functional properties of various glycoproteins, and in the current report it was found that a conservative change of the individual endogenous asparagine residues in CD1d1 to glutamine differentially affected its functional expression. Although the maturation rate of the glycosylation mutants was comparable to that of wild type, they differed in their relative levels of surface expression and in their ability to stimulate NKT cells. Mutating all five glycosylation residues resulted in the absence of detectable CD1d1 expression, with a concomitant lack of NKT cell activation. Therefore, these results demonstrate that glycosylation plays a significant role in the functional expression of CD1d1.

Original languageEnglish (US)
Pages (from-to)272-281
Number of pages10
JournalImmunology
Volume123
Issue number2
DOIs
StatePublished - Feb 1 2008

Keywords

  • Antigen presentation
  • Cell surface expression
  • Glycosylation

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Importance of N-linked glycosylation in the functional expression of murine CD1d1'. Together they form a unique fingerprint.

  • Cite this