Improved autologous cortical bone harvest and viability with 2Flute otologic burs

Adam A. Roth, Pei Ciao Tang, Michael J. Ye, Khalid Mohammad, Rick Nelson

Research output: Contribution to journalArticle

Abstract

Objectives: To determine if 2Flute (Stryker Corporation, Kalamazoo, MI) otologic burs improve the size, cellular content, and bone healing of autologous cortical bone grafts harvested during canal wall reconstruction (CWR) tympanomastoidectomy with mastoid obliteration. Study Design: Institutional review board-approved prospective cohort study. Methods: Human autologous cortical bone chips were harvested using various burs (4 and 6mm diameter; multiflute, and 2Flute [Stryker Corporation]) from patients undergoing CWR tympanomastoidectomy for the treatment of chronic otitis media with cholesteatoma. Bone chip size, cell counts, cellular gene expression, and new bone formation were quantified. Results: Bone chips were significantly larger when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6mm diameter (113±14μm2 vs. 66±8μm2; P<0.05) and 4mm diameter (70±8μm2 vs. 50±3μm2; P<0.05). After 2 weeks in culture, cell numbers were significantly higher when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6mm diameter (48.7±3 vs. 31.8±3 cells/μg bone; P<0.05) and 4mm diameter (27.6±1.2 vs. 8.8±1.2 cells/μg bone; P<0.05). Bone-derived cells express osteoblast markers (alkaline phosphatase, osteocalcin). Cultured cells are able to form new bone in culture, and bone formation is facilitated by the presence of bone chips. Conclusion: Use of 2Flute (Stryker Corporation) otologic burs for human autologous cortical bone harvest results in more viable bone fragments, with larger bone chips and more osteoblasts. Future studies are needed to determine if this leads to improved bone healing. Level of Evidence: NA. Laryngoscope, 2017

Original languageEnglish (US)
JournalLaryngoscope
DOIs
StateAccepted/In press - 2017

Fingerprint

Bone and Bones
Osteoblasts
Osteogenesis
Cortical Bone
Cell Count
Laryngoscopes
Cholesteatoma
Mastoid
Research Ethics Committees
Osteocalcin
Otitis Media
Alkaline Phosphatase
Cultured Cells
Cohort Studies
Prospective Studies
Transplants
Gene Expression

Keywords

  • Autologous
  • Bone
  • Burs
  • Canal wall reconstruction tympanomastoidectomy
  • Cholesteatoma
  • Drill
  • Healing
  • Mastoidectomy
  • Viability

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Improved autologous cortical bone harvest and viability with 2Flute otologic burs. / Roth, Adam A.; Tang, Pei Ciao; Ye, Michael J.; Mohammad, Khalid; Nelson, Rick.

In: Laryngoscope, 2017.

Research output: Contribution to journalArticle

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title = "Improved autologous cortical bone harvest and viability with 2Flute otologic burs",
abstract = "Objectives: To determine if 2Flute (Stryker Corporation, Kalamazoo, MI) otologic burs improve the size, cellular content, and bone healing of autologous cortical bone grafts harvested during canal wall reconstruction (CWR) tympanomastoidectomy with mastoid obliteration. Study Design: Institutional review board-approved prospective cohort study. Methods: Human autologous cortical bone chips were harvested using various burs (4 and 6mm diameter; multiflute, and 2Flute [Stryker Corporation]) from patients undergoing CWR tympanomastoidectomy for the treatment of chronic otitis media with cholesteatoma. Bone chip size, cell counts, cellular gene expression, and new bone formation were quantified. Results: Bone chips were significantly larger when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6mm diameter (113±14μm2 vs. 66±8μm2; P<0.05) and 4mm diameter (70±8μm2 vs. 50±3μm2; P<0.05). After 2 weeks in culture, cell numbers were significantly higher when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6mm diameter (48.7±3 vs. 31.8±3 cells/μg bone; P<0.05) and 4mm diameter (27.6±1.2 vs. 8.8±1.2 cells/μg bone; P<0.05). Bone-derived cells express osteoblast markers (alkaline phosphatase, osteocalcin). Cultured cells are able to form new bone in culture, and bone formation is facilitated by the presence of bone chips. Conclusion: Use of 2Flute (Stryker Corporation) otologic burs for human autologous cortical bone harvest results in more viable bone fragments, with larger bone chips and more osteoblasts. Future studies are needed to determine if this leads to improved bone healing. Level of Evidence: NA. Laryngoscope, 2017",
keywords = "Autologous, Bone, Burs, Canal wall reconstruction tympanomastoidectomy, Cholesteatoma, Drill, Healing, Mastoidectomy, Viability",
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year = "2017",
doi = "10.1002/lary.26779",
language = "English (US)",
journal = "Laryngoscope",
issn = "0023-852X",
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T1 - Improved autologous cortical bone harvest and viability with 2Flute otologic burs

AU - Roth, Adam A.

AU - Tang, Pei Ciao

AU - Ye, Michael J.

AU - Mohammad, Khalid

AU - Nelson, Rick

PY - 2017

Y1 - 2017

N2 - Objectives: To determine if 2Flute (Stryker Corporation, Kalamazoo, MI) otologic burs improve the size, cellular content, and bone healing of autologous cortical bone grafts harvested during canal wall reconstruction (CWR) tympanomastoidectomy with mastoid obliteration. Study Design: Institutional review board-approved prospective cohort study. Methods: Human autologous cortical bone chips were harvested using various burs (4 and 6mm diameter; multiflute, and 2Flute [Stryker Corporation]) from patients undergoing CWR tympanomastoidectomy for the treatment of chronic otitis media with cholesteatoma. Bone chip size, cell counts, cellular gene expression, and new bone formation were quantified. Results: Bone chips were significantly larger when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6mm diameter (113±14μm2 vs. 66±8μm2; P<0.05) and 4mm diameter (70±8μm2 vs. 50±3μm2; P<0.05). After 2 weeks in culture, cell numbers were significantly higher when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6mm diameter (48.7±3 vs. 31.8±3 cells/μg bone; P<0.05) and 4mm diameter (27.6±1.2 vs. 8.8±1.2 cells/μg bone; P<0.05). Bone-derived cells express osteoblast markers (alkaline phosphatase, osteocalcin). Cultured cells are able to form new bone in culture, and bone formation is facilitated by the presence of bone chips. Conclusion: Use of 2Flute (Stryker Corporation) otologic burs for human autologous cortical bone harvest results in more viable bone fragments, with larger bone chips and more osteoblasts. Future studies are needed to determine if this leads to improved bone healing. Level of Evidence: NA. Laryngoscope, 2017

AB - Objectives: To determine if 2Flute (Stryker Corporation, Kalamazoo, MI) otologic burs improve the size, cellular content, and bone healing of autologous cortical bone grafts harvested during canal wall reconstruction (CWR) tympanomastoidectomy with mastoid obliteration. Study Design: Institutional review board-approved prospective cohort study. Methods: Human autologous cortical bone chips were harvested using various burs (4 and 6mm diameter; multiflute, and 2Flute [Stryker Corporation]) from patients undergoing CWR tympanomastoidectomy for the treatment of chronic otitis media with cholesteatoma. Bone chip size, cell counts, cellular gene expression, and new bone formation were quantified. Results: Bone chips were significantly larger when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6mm diameter (113±14μm2 vs. 66±8μm2; P<0.05) and 4mm diameter (70±8μm2 vs. 50±3μm2; P<0.05). After 2 weeks in culture, cell numbers were significantly higher when harvested with 2Flute (Stryker Corporation) bur compared to multiflute burs at both 6mm diameter (48.7±3 vs. 31.8±3 cells/μg bone; P<0.05) and 4mm diameter (27.6±1.2 vs. 8.8±1.2 cells/μg bone; P<0.05). Bone-derived cells express osteoblast markers (alkaline phosphatase, osteocalcin). Cultured cells are able to form new bone in culture, and bone formation is facilitated by the presence of bone chips. Conclusion: Use of 2Flute (Stryker Corporation) otologic burs for human autologous cortical bone harvest results in more viable bone fragments, with larger bone chips and more osteoblasts. Future studies are needed to determine if this leads to improved bone healing. Level of Evidence: NA. Laryngoscope, 2017

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KW - Burs

KW - Canal wall reconstruction tympanomastoidectomy

KW - Cholesteatoma

KW - Drill

KW - Healing

KW - Mastoidectomy

KW - Viability

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