Improved method for the measurement of large neutral amino acids in biological matrices

Rodolfo Bongiovanni, Bryan Yamamoto, George E. Jaskiw

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A high-performance liquid chromatographic method for measuring neutral amino acids in rat sera, brain tissues, and perfusates was developed by using o-phthalaldehyde sulfite as a pre-column derivatization reagent. With the present method, it was possible to separate the neutral amino acids within a single run in 25 min, while the acidic amino acids were eluted near or at the solvent front. The recovery was above 88.8% with a relative standard deviation (RSD) below 4.2%. The within- and between-day assay reproducibility for the determination of rat serum amino acids showed RSDs below 1.35 and 7.61%, respectively. In the present study, the neutral amino acids were assayed with high sensitivity, accuracy and good reproducibility in a relatively short time and on a small sample size.

Original languageEnglish (US)
Pages (from-to)369-376
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume754
Issue number2
DOIs
StatePublished - Apr 25 2001
Externally publishedYes

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Neutral Amino Acids
Rats
o-Phthalaldehyde
Acidic Amino Acids
Sulfites
Assays
Brain
Tissue
Amino Acids
Recovery
Liquids

Keywords

  • Amino acids, neutral
  • Derivatization, LC

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Improved method for the measurement of large neutral amino acids in biological matrices. / Bongiovanni, Rodolfo; Yamamoto, Bryan; Jaskiw, George E.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 754, No. 2, 25.04.2001, p. 369-376.

Research output: Contribution to journalArticle

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