Improved recovery of limb function with ATP/MgCl2 in an ischemic canine hind limb

Robert A. Morgan, Dolores F. Cikrit, Michael Dalsing

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The ability of a solution of low-vanadium-content (less than 1 ppm) adenosine triphosphate and magnesium chloride (ATP/MgCl2) versus normal saline to improve recovery of function and reduce necrosis of skeletal muscle after severe ischemia was investigated in an in situ autoperfused canine hind limb model. The study consisted of 12 dogs divided into 3 study groups: nonischemic control (NIL) (n =7 limbs), ischemic (IL) (n=7 limbs), and ischemic ATP/MgCl2-treated (IATP) (n=7 limbs). In groups IL and IATP, the limb was reperfused for 3 hours following 4 hours of complete ischemia. In IATP limbs, 200μmol/kg of ATP/MgCl2 was infused upon reperfusion of the limbs, whereas IL limbs received a similar volume of normal saline at the time of reperfusion. Function was determined by stimulating the deep peroneal nerve and anterior tibial muscle and measuring the resultant isometric twitch contractile force of paw dorsiflexion. Muscle necrosis was evaluated by photographic analysis of sectioned anterior tibial muscle stained with nitroblue tetrazolium dye. ATP/MgCl2 significantly increased functional recovery (p<0.01) and significantly reduced skeletal muscle necrosis (p<0.01). This study suggests that ATP/MgCl2 may be useful in reducing the clinical sequelae of severe limb ischemia and reperfusion.

Original languageEnglish
Pages (from-to)103-317
Number of pages215
JournalAmerican Journal of Surgery
Volume166
Issue number2
DOIs
StatePublished - 1993
Externally publishedYes

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Recovery of Function
Canidae
Extremities
Adenosine Triphosphate
Skeletal Muscle
Reperfusion
Necrosis
Ischemia
Nitroblue Tetrazolium
Vanadium
Peroneal Nerve
Magnesium Chloride
Coloring Agents
Dogs
Muscles
Control Groups

ASJC Scopus subject areas

  • Surgery

Cite this

Improved recovery of limb function with ATP/MgCl2 in an ischemic canine hind limb. / Morgan, Robert A.; Cikrit, Dolores F.; Dalsing, Michael.

In: American Journal of Surgery, Vol. 166, No. 2, 1993, p. 103-317.

Research output: Contribution to journalArticle

Morgan, Robert A. ; Cikrit, Dolores F. ; Dalsing, Michael. / Improved recovery of limb function with ATP/MgCl2 in an ischemic canine hind limb. In: American Journal of Surgery. 1993 ; Vol. 166, No. 2. pp. 103-317.
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