Improved vectors for nisin-controlled expression in gram-positive bacteria

Edward M. Bryan, Taeok Bae, Michiel Kleerebezem, Gary M. Dunny

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

A set of shuttle vectors, able to replicate in Escherichia coli and in gram-positive bacteria, containing a nisin-inducible promoter (PnisA) and genes encoding NisR and NisK, the two-component signaling mechanism for activating transcription from PnisA in the presence of nisin, was constructed. To test these vectors, Enterococcus faecalis pCF10 plasmid genes prgX, prgY, and prgZ, which respectively encode cytosolic, integral membrane, and cell surface proteins, were cloned downstream of PnisA. Increased protein expression, in the presence of nisin, was demonstrated by Western blot analysis. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)183-190
Number of pages8
JournalPlasmid
Volume44
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Nisin
Gram-Positive Bacteria
Genetic Vectors
Enterococcus faecalis
Genes
Membrane Proteins
Plasmids
Western Blotting
Escherichia coli
Membranes
Proteins

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Improved vectors for nisin-controlled expression in gram-positive bacteria. / Bryan, Edward M.; Bae, Taeok; Kleerebezem, Michiel; Dunny, Gary M.

In: Plasmid, Vol. 44, No. 2, 2000, p. 183-190.

Research output: Contribution to journalArticle

Bryan, Edward M. ; Bae, Taeok ; Kleerebezem, Michiel ; Dunny, Gary M. / Improved vectors for nisin-controlled expression in gram-positive bacteria. In: Plasmid. 2000 ; Vol. 44, No. 2. pp. 183-190.
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