Improving bioassay sensitivity for neurotoxins detection using volterra based third order nonlinear analysis.

Ghassan I. Gholmieh, Spiros H. Courellis, D. Fluster, Lan S. Chen, V. Z. Marmarelis, M. Baudry, T. W. Berger

Research output: Contribution to journalArticle

Abstract

Based on a novel analytical method for analyzing short-term plasticity (STP) of the CA1 hippocampal region in vitro, a screening tool for the detection and classification of unknown chemical compounds affecting the nervous system was recently introduced [1], [2]. The recorded signal consisted of evoked population spike in response to Poisson distributed random train impulse stimuli. The developed analytical approach used the first order Volterra kernel and the Laguerre coefficients of the second order Volterra model as classification features [3]. The biosensor showed encouraging results, and was able to classify out of sample compounds correctly [2]. We have taken an exploratory step to investigate the advantage of introducing a third order model [4]. DAP5, an NMDA channel blocker, did not show major changes in the second order kernel and in its corresponding Laguerre coefficients. Data were reanalyzed using a third order model. DAP5 showed discernable changes in the third order kernel as well as in the some of the corresponding Laguerre coefficients. Hence, the third order Volterra based model has the potential to improve the sensitivity and the discriminatory power of the proposed bioassay.

Original languageEnglish (US)
Pages (from-to)2261-2264
Number of pages4
JournalConference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference
StatePublished - 2007
Externally publishedYes

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Bioassay
Neurotoxins
Nonlinear analysis
Biological Assay
Hippocampal CA1 Region
Biosensing Techniques
N-Methylaspartate
Nervous System
Chemical compounds
Neurology
Biosensors
Population
Plasticity
Screening
In Vitro Techniques

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

Cite this

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title = "Improving bioassay sensitivity for neurotoxins detection using volterra based third order nonlinear analysis.",
abstract = "Based on a novel analytical method for analyzing short-term plasticity (STP) of the CA1 hippocampal region in vitro, a screening tool for the detection and classification of unknown chemical compounds affecting the nervous system was recently introduced [1], [2]. The recorded signal consisted of evoked population spike in response to Poisson distributed random train impulse stimuli. The developed analytical approach used the first order Volterra kernel and the Laguerre coefficients of the second order Volterra model as classification features [3]. The biosensor showed encouraging results, and was able to classify out of sample compounds correctly [2]. We have taken an exploratory step to investigate the advantage of introducing a third order model [4]. DAP5, an NMDA channel blocker, did not show major changes in the second order kernel and in its corresponding Laguerre coefficients. Data were reanalyzed using a third order model. DAP5 showed discernable changes in the third order kernel as well as in the some of the corresponding Laguerre coefficients. Hence, the third order Volterra based model has the potential to improve the sensitivity and the discriminatory power of the proposed bioassay.",
author = "Gholmieh, {Ghassan I.} and Courellis, {Spiros H.} and D. Fluster and Chen, {Lan S.} and Marmarelis, {V. Z.} and M. Baudry and Berger, {T. W.}",
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AU - Gholmieh, Ghassan I.

AU - Courellis, Spiros H.

AU - Fluster, D.

AU - Chen, Lan S.

AU - Marmarelis, V. Z.

AU - Baudry, M.

AU - Berger, T. W.

PY - 2007

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N2 - Based on a novel analytical method for analyzing short-term plasticity (STP) of the CA1 hippocampal region in vitro, a screening tool for the detection and classification of unknown chemical compounds affecting the nervous system was recently introduced [1], [2]. The recorded signal consisted of evoked population spike in response to Poisson distributed random train impulse stimuli. The developed analytical approach used the first order Volterra kernel and the Laguerre coefficients of the second order Volterra model as classification features [3]. The biosensor showed encouraging results, and was able to classify out of sample compounds correctly [2]. We have taken an exploratory step to investigate the advantage of introducing a third order model [4]. DAP5, an NMDA channel blocker, did not show major changes in the second order kernel and in its corresponding Laguerre coefficients. Data were reanalyzed using a third order model. DAP5 showed discernable changes in the third order kernel as well as in the some of the corresponding Laguerre coefficients. Hence, the third order Volterra based model has the potential to improve the sensitivity and the discriminatory power of the proposed bioassay.

AB - Based on a novel analytical method for analyzing short-term plasticity (STP) of the CA1 hippocampal region in vitro, a screening tool for the detection and classification of unknown chemical compounds affecting the nervous system was recently introduced [1], [2]. The recorded signal consisted of evoked population spike in response to Poisson distributed random train impulse stimuli. The developed analytical approach used the first order Volterra kernel and the Laguerre coefficients of the second order Volterra model as classification features [3]. The biosensor showed encouraging results, and was able to classify out of sample compounds correctly [2]. We have taken an exploratory step to investigate the advantage of introducing a third order model [4]. DAP5, an NMDA channel blocker, did not show major changes in the second order kernel and in its corresponding Laguerre coefficients. Data were reanalyzed using a third order model. DAP5 showed discernable changes in the third order kernel as well as in the some of the corresponding Laguerre coefficients. Hence, the third order Volterra based model has the potential to improve the sensitivity and the discriminatory power of the proposed bioassay.

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