In situ Effect of Arginine-Containing Dentifrice on Plaque Composition and on Enamel Demineralization under Distinct Cariogenic Conditions

Angel Y. Sanchez, Caroline L. de Oliveira, Thais C. Negrini, Lina N. Hashizume, Anderson Hara, Marisa Maltz, Rodrigo A. Arthur

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

There is limited evidence that arginine-containing fluoridated dentifrices (AFD) have a better anticaries effect than regular fluoridated dentifrices (FD), especially in subjects at a higher risk for caries development. This study aimed to assess the effect of AFD on enamel demineralization and on the microbial and biochemical compositions of biofilm formed under different frequencies of sucrose exposure. It consisted of an in situ split-mouth design, where 12 adult volunteers who used FD for at least 2 months prior to the beginning of this study wore acrylic palatal appliances containing 4 bovine enamel specimens (1 pair at each side of the appliance) during 2 phases of 14 days each. FD slurry (3×/day) and 20% sucrose solution (4× and 8×/day) were dripped on the specimens during the first experimental phase. The same volunteers then used AFD during a 2-month washout period, followed by a second experimental phase where the AFD slurry and sucrose solution were applied onto a new subset of specimens. The percentage of enamel surface hardness loss (%SHL), the lesion depth (LD), the integrated mineral loss (IML), microbial counts on biofilms, the biomass, and inorganic and insoluble extracellular polysaccharide (IEPS) biofilm concentrations were determined. Higher %SHL, biomass, and IEPS and lower fluoride values were found at sucrose 8×/day exposure. Lower IEPS were found in the presence of AFD compared to FD. Similar %SHL, LD, and IML values were found between FD and AFD, irrespectively of the cariogenic challenge. The results suggest that AFD have an anticaries effect similar to that of regular FD.

Original languageEnglish (US)
Pages (from-to)588-597
Number of pages10
JournalCaries Research
DOIs
StateAccepted/In press - May 4 2018

Fingerprint

Dentifrices
Dental Enamel
Arginine
Sucrose
Biofilms
Polysaccharides
Biomass
Minerals
Volunteers
Hardness
Fluorides

Keywords

  • Arginine
  • Demineralization
  • Dental caries
  • Dentifrice
  • Enamel
  • Fluoride
  • Sucrose

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Sanchez, A. Y., de Oliveira, C. L., Negrini, T. C., Hashizume, L. N., Hara, A., Maltz, M., & Arthur, R. A. (Accepted/In press). In situ Effect of Arginine-Containing Dentifrice on Plaque Composition and on Enamel Demineralization under Distinct Cariogenic Conditions. Caries Research, 588-597. https://doi.org/10.1159/000488212

In situ Effect of Arginine-Containing Dentifrice on Plaque Composition and on Enamel Demineralization under Distinct Cariogenic Conditions. / Sanchez, Angel Y.; de Oliveira, Caroline L.; Negrini, Thais C.; Hashizume, Lina N.; Hara, Anderson; Maltz, Marisa; Arthur, Rodrigo A.

In: Caries Research, 04.05.2018, p. 588-597.

Research output: Contribution to journalArticle

Sanchez, Angel Y. ; de Oliveira, Caroline L. ; Negrini, Thais C. ; Hashizume, Lina N. ; Hara, Anderson ; Maltz, Marisa ; Arthur, Rodrigo A. / In situ Effect of Arginine-Containing Dentifrice on Plaque Composition and on Enamel Demineralization under Distinct Cariogenic Conditions. In: Caries Research. 2018 ; pp. 588-597.
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