In situ hybridization analysis of Girk2 expression in the developing central nervous system in normal and weaver mice

Jianjun Wei, Stephen R. Dlouhy, Shirley Bayer, Roberto Piva, Tatyana Verina, Yi Wang, Yue Feng, Brenda Dupree, M. E. Hodes, Bernardino Ghetti

Research output: Contribution to journalReview article

25 Scopus citations

Abstract

A mutation in the gene Girk2 that encodes an inwardly rectifying potassium channel is the genetic defect caesing the behavioral and pathologic abnormalities of the weaver mutant mouse. Of the pathologic abnormalities, the best stud ted is the neuronal degeneration that occurs in the cerebellar cortex and in the midbrain dopaminergic neurons. A detailed characterization of the topographic and temporal expression of Girk2 is fundamental to elucidate the mechanisms underlying neurodegeneration in these mutant mice. In this study we utilized in situ hybridization to determine the expression of Girk2 mRNA during prenatal and postnatal development in the murine central nervous system (CNS). Girk2 expression was seen in multiple regions of embryonic CNS including the cerebellum and midbrain. During postnatal development, the highest expression was seen in the cerebellum, midbrain and hippocampus. However, since the developing cerebellum undergoes significant neuronal loss due to the degeneration of granule cell precursors. Girk2 mRNA expression in this area decreases progressively.

Original languageEnglish (US)
Pages (from-to)762-771
Number of pages10
JournalJournal of Neuropathology and Experimental Neurology
Volume56
Issue number7
DOIs
StatePublished - Jul 1997

Keywords

  • Girk2
  • In situ hybridization
  • Potassium channel
  • Weaver

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

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