In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain.

Kathryn Jones, D. M. Chikaraishi, C. A. Harrington, B. S. McEwen, D. W. Pfaff

Research output: Contribution to journalArticle

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Abstract

In this study, quantitative assessment of estradiol (E2)-induced changes in levels of ribosomal RNA within brain regions concentrating the hormone was accomplished by in situ hybridization with nick-translated tritiated ribosomal DNA probes and use of a computer-based image analysis system. Ovariectomized rats were either implanted with estradiol capsules for 6 h, 24 h, or 15 days, or sham-implanted under the same time course to serve as controls. The mean number of grains, somal area, and grain density of neurons within three E2-concentrating brain regions, the ventrolateral portion of the ventromedial and the arcuate nuclei of the hypothalamus (VL-VMN and ARC, respectively) and the corticomedial nucleus of the amygdala (AMY) were determined. In the VL-VMN and ARC, levels of rRNA were significantly increased after 6 h of E2 treatment (70% and 30%, respectively) and after 24 h of E2 treatment (60% and 62%, respectively). However, these effects on rRNA levels in VL-VMN and ARC were not observed after prolonged exposure of 15 days to the hormone. Neuronal hypertrophy was present only after 24 h of E2 treatment in the VL-VMN and ARC (32% and 14%, respectively). No changes were found in the AMY. As an additional internal control, measurements were also collected from the dorsomedial portion of the VMN (DM-VMN), a region with few E2-concentrating neurons. No changes in any of the parameters were found in DM-VMN at any time after exposure to the hormone. By extending the in situ hybridization technique to the quantitative level, these findings demonstrate differential estrogenic regulation of a known gene product, rRNA, in rat brain that is temporally and regionally specific.

Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalBrain Research
Volume387
Issue number2
StatePublished - Nov 1986
Externally publishedYes

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Ribosomal RNA
In Situ Hybridization
Estradiol
Hormones
Amygdala
Brain
Neurons
Arcuate Nucleus of Hypothalamus
DNA Probes
Ribosomal DNA
rRNA Genes
Hypertrophy
Capsules

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Jones, K., Chikaraishi, D. M., Harrington, C. A., McEwen, B. S., & Pfaff, D. W. (1986). In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain. Brain Research, 387(2), 145-152.

In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain. / Jones, Kathryn; Chikaraishi, D. M.; Harrington, C. A.; McEwen, B. S.; Pfaff, D. W.

In: Brain Research, Vol. 387, No. 2, 11.1986, p. 145-152.

Research output: Contribution to journalArticle

Jones, K, Chikaraishi, DM, Harrington, CA, McEwen, BS & Pfaff, DW 1986, 'In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain.', Brain Research, vol. 387, no. 2, pp. 145-152.
Jones K, Chikaraishi DM, Harrington CA, McEwen BS, Pfaff DW. In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain. Brain Research. 1986 Nov;387(2):145-152.
Jones, Kathryn ; Chikaraishi, D. M. ; Harrington, C. A. ; McEwen, B. S. ; Pfaff, D. W. / In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain. In: Brain Research. 1986 ; Vol. 387, No. 2. pp. 145-152.
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