In vitro and in vivo measurement of red cell velocity with epi- and transillumination

Scot L. Harper, H. Bohlen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Measurement of red cell velocity with the dual-slit cross-correlation method in glass capillary tubes during transillumination indicates that the measured velocity must be divided by a correction factor of approximately 1.6 to equal the average velocity calculated from a known flow and inner diameter. Whether the same correction factor exists when red cell velocity is measured during epiillumination is questionable. Red cell velocity was measured with the dual-slit correlation method nearly simultaneously using epi- (EL) and transillumination (TL) while glass tubes (40-100 μm, i.d.) were pump perfused with whole human blood (hemotocrit 39-42%). With TL, the measured velocity is 1.58 ± 0.07 (SEM) times the calculated average velocity, whereas a factor of 2.04 ± 0.04 (SEM) was obtained with epiillumination. When intestinal arterioles with approximately the same inner diameters and flow velocities as the glass tubes were used, the ratio of velocities measured with TL to EL was 1.21 ± 0.02 (SEM) as compared to 1.31 ± 0.09 (SEM) for glass tubes using TL and EL of the tube at the same pump flow. This similarity of TL to EL velocity ratios for glass tubes and microvessels may be fortuitous or indicate that comparable flow properties and measurement conditions exist for in vitro and in vivo situations. The major finding of the study is, however, that different velocity correction factors exist for EL and TL measurements when the dual-slit correlation method is used to estimate red cell velocities in tubes of an internal diameter of 40-100 μm at normal hematocrits.

Original languageEnglish
Pages (from-to)186-193
Number of pages8
JournalMicrovascular Research
Volume25
Issue number2
DOIs
StatePublished - 1983

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Transillumination
Cells
Glass
Correlation methods
Scanning electron microscopy
Arterioles
Microvessels
In Vitro Techniques
Hematocrit
Pumps
Capillary tubes
Flow velocity

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

In vitro and in vivo measurement of red cell velocity with epi- and transillumination. / Harper, Scot L.; Bohlen, H.

In: Microvascular Research, Vol. 25, No. 2, 1983, p. 186-193.

Research output: Contribution to journalArticle

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