In Vitro Construction of 2D and 3D simulations of the murine hematopoietic niche

Brahmananda Reddy Chitteti, Monique Bethel, Sherry L. Voytik-Harbin, Melissa Kacena, Edward Srour

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Hematopoietic stem cells (HSC) undergo multilineage differentiation or self-renewal to maintain normal hematopoiesis and to sustain the size of the HSC pool throughout life. These processes are determined by a complex interplay of molecular signals between HSC and other cellular components such as osteoblasts (OB), stromal cells, endothelial cells, and a number of extracellular matrix (ECM) proteins. Through changes in its physical properties within the bone marrow (BM) microenvironment, collagen, which is one of the most critical ECM proteins, can modulate HSC function and maintenance of the competence of the hematopoietic niche (HN). At present, there is no consensus as to how different cellular elements of the niche collaborate and interact to promote HSC self-renewal or differentiation to maintain hematopoiesis. Deciphering these interactions and the impact of mechanical properties of the collagen microstructures within the HN has critical clinical implications in the areas of stem cell homing, engraftment, and maintenance of HSC function. In this chapter, we describe several of the in vitro methodologies for establishing and maintaining HSC in vitro including the isolation of OB, stromal cells, and hematopoietic progenitor cells, as well as the establishment of both two-dimensional (2D) and three-dimensional (3D) coculture systems.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages43-56
Number of pages14
Volume1035
ISBN (Print)9781627035071
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1035
ISSN (Print)10643745

Fingerprint

Hematopoietic Stem Cells
Extracellular Matrix Proteins
Hematopoiesis
Stromal Cells
Osteoblasts
Collagen
Maintenance
In Vitro Techniques
Coculture Techniques
Mental Competency
Consensus
Stem Cells
Endothelial Cells
Cell Count
Bone Marrow

Keywords

  • Collagen matrix
  • Hematopoietic stem cells
  • Osteoblasts
  • Stem cell niche
  • Stromal cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Reddy Chitteti, B., Bethel, M., Voytik-Harbin, S. L., Kacena, M., & Srour, E. (2013). In Vitro Construction of 2D and 3D simulations of the murine hematopoietic niche. In Methods in Molecular Biology (Vol. 1035, pp. 43-56). (Methods in Molecular Biology; Vol. 1035). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-508-8_5

In Vitro Construction of 2D and 3D simulations of the murine hematopoietic niche. / Reddy Chitteti, Brahmananda; Bethel, Monique; Voytik-Harbin, Sherry L.; Kacena, Melissa; Srour, Edward.

Methods in Molecular Biology. Vol. 1035 Humana Press Inc., 2013. p. 43-56 (Methods in Molecular Biology; Vol. 1035).

Research output: Chapter in Book/Report/Conference proceedingChapter

Reddy Chitteti, B, Bethel, M, Voytik-Harbin, SL, Kacena, M & Srour, E 2013, In Vitro Construction of 2D and 3D simulations of the murine hematopoietic niche. in Methods in Molecular Biology. vol. 1035, Methods in Molecular Biology, vol. 1035, Humana Press Inc., pp. 43-56. https://doi.org/10.1007/978-1-62703-508-8_5
Reddy Chitteti B, Bethel M, Voytik-Harbin SL, Kacena M, Srour E. In Vitro Construction of 2D and 3D simulations of the murine hematopoietic niche. In Methods in Molecular Biology. Vol. 1035. Humana Press Inc. 2013. p. 43-56. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-508-8_5
Reddy Chitteti, Brahmananda ; Bethel, Monique ; Voytik-Harbin, Sherry L. ; Kacena, Melissa ; Srour, Edward. / In Vitro Construction of 2D and 3D simulations of the murine hematopoietic niche. Methods in Molecular Biology. Vol. 1035 Humana Press Inc., 2013. pp. 43-56 (Methods in Molecular Biology).
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