In vitro contractile effects of neurokinin receptor blockade in the human ureter

Stephen Y. Nakada, Travis Jerde, Dale E. Bjorling, Ricardo Saban

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose: We identified the predominance of neurokinin-2 receptors and evaluated the inhibition of spontaneous contraction via the blockade of neurokinin-2 receptors in human ureteral segments. Materials and Methods: Excess ureteral segments from human subjects undergoing donor nephrectomy or reconstructive procedures were suspended in tissue baths containing Krebs buffer. After spontaneous contractions were recorded, tissues were incubated with 1 μM. solutions of phosphoramidon and captopril (to inhibit peptide degradation) and either the neurokinin-1 receptor antagonist CP 99,994, the neurokinin-2 receptor antagonist SR 48,968, the neurokinin-3 receptor antagonist SR 142,801 or dimethyl sulfoxide (control) for 1 hour. Contraction magnitude and frequency were again recorded and compared with spontaneous levels. Concentration-response curves to the tachykinins substance P, and neurokinins A and B were determined in the presence and absence of antagonists. Results: Neurokinin A increased contractility at lower concentrations than substance P or neurokinin B (p

Original languageEnglish (US)
Pages (from-to)1534-1538
Number of pages5
JournalJournal of Urology
Volume166
Issue number4
StatePublished - 2001
Externally publishedYes

Fingerprint

Neurokinin-2 Receptors
Ureter
Neurokinin B
Neurokinin A
Substance P
Neurokinin-3 Receptors
Neurokinin-1 Receptor Antagonists
Tachykinins
Captopril
Dimethyl Sulfoxide
Nephrectomy
Baths
Buffers
Peptides
In Vitro Techniques

Keywords

  • Muscle
  • Muscle contraction
  • Neurokinin-2
  • Receptors
  • Smooth
  • Ureter

ASJC Scopus subject areas

  • Urology

Cite this

Nakada, S. Y., Jerde, T., Bjorling, D. E., & Saban, R. (2001). In vitro contractile effects of neurokinin receptor blockade in the human ureter. Journal of Urology, 166(4), 1534-1538.

In vitro contractile effects of neurokinin receptor blockade in the human ureter. / Nakada, Stephen Y.; Jerde, Travis; Bjorling, Dale E.; Saban, Ricardo.

In: Journal of Urology, Vol. 166, No. 4, 2001, p. 1534-1538.

Research output: Contribution to journalArticle

Nakada, SY, Jerde, T, Bjorling, DE & Saban, R 2001, 'In vitro contractile effects of neurokinin receptor blockade in the human ureter', Journal of Urology, vol. 166, no. 4, pp. 1534-1538.
Nakada, Stephen Y. ; Jerde, Travis ; Bjorling, Dale E. ; Saban, Ricardo. / In vitro contractile effects of neurokinin receptor blockade in the human ureter. In: Journal of Urology. 2001 ; Vol. 166, No. 4. pp. 1534-1538.
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