In vitro cytotoxicity of silver-impregnated collagen cuffs designed to decrease infection in tunneled catheters

Jeffrey B. Hemmerlein, Scott O. Trerotola, Michael Kraus, Marc Mendonca, Lael A. Desmond

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

PURPOSE: To examine in vitro the effects of silver-impregnated collagen cuff material from central venous catheters on human fibroblast growth. MATERIALS AND METHODS: In culture flasks, hybrid cells were exposed to silver-impregnated collagen cuff material, and human fibroblasts were exposed to silver-impregnated or silver-free collagen cuff material. After 72 hours of growth, cells were stained and digitally imaged, and the relative areas of cytotoxicity were determined. RESULTS: Flasks containing the silver- impregnated collagen cuff material and hybrid cells or human fibroblasts showed a marked local cytotoxic effect of the cuff material; cell-free zones surrounding the cuff material were demonstrated. No cytotoxic effect was seen in the flasks that contained silver-free cuff material (control group). Mean area of cleared cells was 312 mm2 ± 130 (range, 156624 mm2) in the flasks containing human fibroblasts and silver-impregnated cuff material and 0 mm2 in the corresponding control flasks (P < .0001). Mean radius of the area of cleared cells around the silver impregnated cuff material in the flasks containing human fibroblasts was 9.8 mm ± 2.0 (range, 7.0-14.1 mm). CONCLUSION: Silver-impregnated collagen cuff material demonstrates a local cytotoxicity on hybrid cells and human fibroblasts in vitro. This finding may explain the phenomena seen clinically of decreased anchorage and inadvertent removal of catheters with silver-impregnated collagen cuffs.

Original languageEnglish
Pages (from-to)363-367
Number of pages5
JournalRadiology
Volume204
Issue number2
StatePublished - Aug 1997

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Silver
Collagen
Catheters
Infection
Fibroblasts
Hybrid Cells
In Vitro Techniques
Central Venous Catheters
Growth
Control Groups

Keywords

  • Catheters and catheterization, central venous access
  • Catheters and catheterization, complications

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Hemmerlein, J. B., Trerotola, S. O., Kraus, M., Mendonca, M., & Desmond, L. A. (1997). In vitro cytotoxicity of silver-impregnated collagen cuffs designed to decrease infection in tunneled catheters. Radiology, 204(2), 363-367.

In vitro cytotoxicity of silver-impregnated collagen cuffs designed to decrease infection in tunneled catheters. / Hemmerlein, Jeffrey B.; Trerotola, Scott O.; Kraus, Michael; Mendonca, Marc; Desmond, Lael A.

In: Radiology, Vol. 204, No. 2, 08.1997, p. 363-367.

Research output: Contribution to journalArticle

Hemmerlein, JB, Trerotola, SO, Kraus, M, Mendonca, M & Desmond, LA 1997, 'In vitro cytotoxicity of silver-impregnated collagen cuffs designed to decrease infection in tunneled catheters', Radiology, vol. 204, no. 2, pp. 363-367.
Hemmerlein, Jeffrey B. ; Trerotola, Scott O. ; Kraus, Michael ; Mendonca, Marc ; Desmond, Lael A. / In vitro cytotoxicity of silver-impregnated collagen cuffs designed to decrease infection in tunneled catheters. In: Radiology. 1997 ; Vol. 204, No. 2. pp. 363-367.
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N2 - PURPOSE: To examine in vitro the effects of silver-impregnated collagen cuff material from central venous catheters on human fibroblast growth. MATERIALS AND METHODS: In culture flasks, hybrid cells were exposed to silver-impregnated collagen cuff material, and human fibroblasts were exposed to silver-impregnated or silver-free collagen cuff material. After 72 hours of growth, cells were stained and digitally imaged, and the relative areas of cytotoxicity were determined. RESULTS: Flasks containing the silver- impregnated collagen cuff material and hybrid cells or human fibroblasts showed a marked local cytotoxic effect of the cuff material; cell-free zones surrounding the cuff material were demonstrated. No cytotoxic effect was seen in the flasks that contained silver-free cuff material (control group). Mean area of cleared cells was 312 mm2 ± 130 (range, 156624 mm2) in the flasks containing human fibroblasts and silver-impregnated cuff material and 0 mm2 in the corresponding control flasks (P < .0001). Mean radius of the area of cleared cells around the silver impregnated cuff material in the flasks containing human fibroblasts was 9.8 mm ± 2.0 (range, 7.0-14.1 mm). CONCLUSION: Silver-impregnated collagen cuff material demonstrates a local cytotoxicity on hybrid cells and human fibroblasts in vitro. This finding may explain the phenomena seen clinically of decreased anchorage and inadvertent removal of catheters with silver-impregnated collagen cuffs.

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