In vitro drug screening and individualized treatment planning for cancer patients.

M. T. Tseng, Ahmad Safa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have developed an in vitro screening test for anticancer agents, using monodispersed tumor cells derived from 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary tumors in rat. Monolayer cell cultures in multiwell plates are maintained in medium supplemented with hormones and fetal calf serum. Guided by a randomized table, drugs are administered to cultures singly or in combination. Thus far, we have defined dose-response curves for tamoxifen, adriamycin, thiotepa, and methotrexate. Of these agents, adriamycin consistently exhibits a greater cytocidal effect for a given concentration. When these agents are given in combination, a general enhancement of cytotoxicity is observed, although the effects are frequently not additive. Using this procedure, we have examined the drug sensitivity of cells from human tumor biopsies. Data are being compiled for retrospective comparison with the results of treatment for these patients. The procedure described is suitable for analysis of most solid tumors; the evaluation can be completed within 1-2 weeks. Ease of cell maintenance and the brief incubation required suggest that this system may be suitable for general clinical use.

Original languageEnglish
Pages (from-to)371-379
Number of pages9
JournalCancer Detection and Prevention
Volume6
Issue number3
StatePublished - 1983
Externally publishedYes

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Preclinical Drug Evaluations
Doxorubicin
Thiotepa
Neoplasms
9,10-Dimethyl-1,2-benzanthracene
Tamoxifen
Methotrexate
Pharmaceutical Preparations
Antineoplastic Agents
Therapeutics
Cell Culture Techniques
Maintenance
Hormones
Breast Neoplasms
Biopsy
Serum
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vitro drug screening and individualized treatment planning for cancer patients. / Tseng, M. T.; Safa, Ahmad.

In: Cancer Detection and Prevention, Vol. 6, No. 3, 1983, p. 371-379.

Research output: Contribution to journalArticle

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