In vitro effects of sports and energy drinks on streptococcus mutans biofilm formation and metabolic activity

LaQuia Vinson, Amy K. Goodlett, Ruijie Huang, George J. Eckert, Richard Gregory

Research output: Contribution to journalArticle

Abstract

Purpose: Sports and energy drinks are being increasingly consumed and contain large amounts of sugars, which are known to increase Streptococcus mutans biofilm formation and metabolic activity. The purpose of this in vitro study was to investigate the effects of sports and energy drinks on S. mutans biofilm formation and metabolic activity. Methods: S. mutans UA159 was cultured with and without a dilution (1:3 ratio) of a variety of sports and energy drinks in bacterial media for 24 hours. The biofilm was washed, fixed, and stained. Biofilm growth was evaluated by reading absorbance of the crystal violet. Biofilm metabolic activity was measured by the biofilm-reducing XTT to a water-soluble orange compound. Results: Gatorade Protein Recovery Shake and Starbucks Doubleshot Espresso Energy were found to significantly increase biofilm (30-fold and 22-fold, respectively) and metabolic activity (2-fold and 3-fold, respectively). However, most of the remaining drinks significantly inhibited biofilm growth and metabolic activity. Conclusions: Several sports and energy drinks, with sugars or sugar substitutes as their main ingredients inhibited S. mutans biofilm formation. Among the drinks evaluated, Gatorade Protein Recovery Chocolate Shake and Starbucks Doubleshot Energy appear to have cariogenic potential since they increased the biofilm formation and metabolic activity of S. mutans.

Original languageEnglish (US)
Pages (from-to)108-114
Number of pages7
JournalJournal of Dentistry for Children
Volume84
Issue number3
StatePublished - Sep 1 2017

Fingerprint

Energy Drinks
Streptococcus mutans
Biofilms
Sports
In Vitro Techniques
Gentian Violet
Sweetening Agents
Growth
Reading
Proteins

Keywords

  • Biofilm
  • Energy drinks
  • Sports drinks
  • Streptococcus mutans

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

In vitro effects of sports and energy drinks on streptococcus mutans biofilm formation and metabolic activity. / Vinson, LaQuia; Goodlett, Amy K.; Huang, Ruijie; Eckert, George J.; Gregory, Richard.

In: Journal of Dentistry for Children, Vol. 84, No. 3, 01.09.2017, p. 108-114.

Research output: Contribution to journalArticle

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