In vitro histotypic reaggregation of fetal rat lung cells following cryopreservation and storage in liquid nitrogen

J. A. McAteer, W. H J Douglas

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Enzymatically dissociated fetal rat lung cells were frozen in medium containing dimethyl sulfoxide (DMSO) as the cryoprotectant, and stored at liquid nitrogen temperature. The cells were retrieved from frozen storage and seeded into Gelfoam collagen sponges. The cultures were examined morphologically using tetrazolium chloride (TTC) vital staining, light microscopy, and transmission electron microscopy, at various points throughout a two-week in vitro period. Fetal rat lung organotypic cultures established from previously frozen cells possessed the characteristics of cultures derived from freshly dissociated lung. Though the percent recovery of viable cells following freezing was low (

Original languageEnglish (US)
Pages (from-to)73-83
Number of pages11
JournalBirth Defects: Original Article Series
Volume16
Issue number2
StatePublished - 1980
Externally publishedYes

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Cryopreservation
Nitrogen
Lung
Absorbable Gelatin Sponge
Porifera
Dimethyl Sulfoxide
Transmission Electron Microscopy
Freezing
Chlorides
Microscopy
Collagen
Staining and Labeling
Light
Temperature
In Vitro Techniques

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics(clinical)

Cite this

In vitro histotypic reaggregation of fetal rat lung cells following cryopreservation and storage in liquid nitrogen. / McAteer, J. A.; Douglas, W. H J.

In: Birth Defects: Original Article Series, Vol. 16, No. 2, 1980, p. 73-83.

Research output: Contribution to journalArticle

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