In vitro induction of hydrolytic activity in human gingival and pulp fibroblasts by triethylene glycol dimethacrylate and monocyte chemotatic protein-1

Karen S. Gregson, J. Terrence O'Neill, Jeffrey Platt, L. Windsor

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: Some dental resin composite materials leach the monomer triethylene glycol dimethacrylate (TEGDMA), which could contribute to the inflammatory reaction. The purpose of this study was to examine the ability of TEGDMA to alter cytokine/growth factor secretion and enzymatic activity in monocyte derived macrophages (U937 cells), human gingival fibroblasts (HGFs), and human pulp fibroblasts (DPFs). Methods: A human growth factor/cytokine antibody array was utilized to determine whether TEGDMA alters the expression of cytokines/growth factors from U937 cells, HGFs, and DPFs. To determine if TEGDMA alters the hydrolase activity of U937, DPF, and HGF cells, the hydrolysis of p-nitrophenyl butyrate was utilized in a spectrophotometric assay. Results: TEGDMA exposure induced the expression of monocyte chemotatic protein-1 (MCP-1) from the U937 cells. Both the cell lysates and conditioned media from the HGFs showed increased hydrolase activity after exposure to a sublethal concentration of TEGDMA. MCP-1 alone increased the hydrolytic activity and the combination of MCP-1 and TEGDMA was additive in HGF conditioned media. The DPFs were also exposed to MCP-1 alone and followed by a sublethal concentration of TEGDMA. The effect of MCP-1 was greater than that of TEGDMA. Significance: These results demonstrate that TEGDMA induces enzymatic activity and cytokine/growth factor expression in a cell-specific manner.

Original languageEnglish (US)
Pages (from-to)1461-1467
Number of pages7
JournalDental Materials
Volume24
Issue number11
DOIs
StatePublished - Nov 2008

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Fibroblasts
Glycols
Human Activities
Pulp
Monocytes
Proteins
U937 Cells
Intercellular Signaling Peptides and Proteins
Cytokines
Hydrolases
Conditioned Culture Medium
In Vitro Techniques
triethylene glycol dimethacrylate
Macrophages
Antibodies
Hydrolysis
Assays
Resins
Monomers
Composite materials

Keywords

  • Dental materials
  • Human gingival fibroblasts
  • Human pulp fibroblasts
  • Monocyte chemotatic protein-1

ASJC Scopus subject areas

  • Dentistry(all)
  • Materials Science(all)
  • Mechanics of Materials

Cite this

In vitro induction of hydrolytic activity in human gingival and pulp fibroblasts by triethylene glycol dimethacrylate and monocyte chemotatic protein-1. / Gregson, Karen S.; Terrence O'Neill, J.; Platt, Jeffrey; Windsor, L.

In: Dental Materials, Vol. 24, No. 11, 11.2008, p. 1461-1467.

Research output: Contribution to journalArticle

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