In vitro inhibition of cellular immune reactions by herbicolin and streptomyces extract

B. Maier, D. Baron, W. Bessler

Research output: Contribution to journalArticle

Abstract

Herbicolin, an extremely lipophilic cyclic heptapeptide secreted from Erwinia herbicola, inhibits human allogeneic mixed leukocyte culture up to more than 90% at a concentration of 5 μg/ml. Under these experimental conditions, the generation of cytotoxic T-lymphocytes is completely suppressed. The inhibition strongly depends on the solvent used. Herbicolin is a priori non-toxic to human lymphocytes and it does not alter the antigenic properties of the HLA-A,B,C and DR antigens. The soluble extract of mycelia of Streptomyces has two different effects on human peripheral blood lymphocytes: it is a mitogen and it inhibits the mixed leukocyte. The latter property resides in the induction of suppressor cells which show their highest activity after 3 days of culture. The suppressor cells consist of radiolabile and radioresistant subpopulations. The cell-free supernatant of cultures used to generate suppressor cells does not show any inhibitory activity.

Original languageEnglish (US)
Pages (from-to)31-35
Number of pages5
JournalLangenbecks Archiv fur Chirurgie
Volume356
Issue numberSuppl. 1982
StatePublished - Jan 1 1982

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ASJC Scopus subject areas

  • Surgery

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