Herbicolin, an extremely lipophilic cyclic heptapeptide secreted from Erwinia herbicola, inhibits human allogeneic mixed leukocyte culture up to more than 90% at a concentration of 5 μg/ml. Under these experimental conditions, the generation of cytotoxic T-lymphocytes is completely suppressed. The inhibition strongly depends on the solvent used. Herbicolin is a priori non-toxic to human lymphocytes and it does not alter the antigenic properties of the HLA-A,B,C and DR antigens. The soluble extract of mycelia of Streptomyces has two different effects on human peripheral blood lymphocytes: it is a mitogen and it inhibits the mixed leukocyte. The latter property resides in the induction of suppressor cells which show their highest activity after 3 days of culture. The suppressor cells consist of radiolabile and radioresistant subpopulations. The cell-free supernatant of cultures used to generate suppressor cells does not show any inhibitory activity.
|Original language||English (US)|
|Number of pages||5|
|Journal||Langenbecks Archiv fur Chirurgie|
|Issue number||Suppl. 1982|
|State||Published - Jan 1 1982|
ASJC Scopus subject areas