In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors

S. Pazzaglia, X. R. Chen, C. B. Aamodt, S. Q. Wu, Chinghai Kao, K. W. Gilchrist, R. Oyasu, C. A. Reznikoff, M. A. Ritter

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Recent interest has focused on the identification of molecular genetic mechanisms in multistep neoplastic transformation. In vitro exposure of simian virus 40 (SV40)-immortalized human uroepithelial cells (SV-HUC) that are environmentally relevant to bladder carcinogens has been shown to produce tumorigenic transformation, as assessed by the ability of cells exposed to a carcinogen to form xenograph tumors with heterogeneous cancer phenotypes ranging from very aggressive, invasive high-grade carcinomas to superficial low-grade indolent tumors. In addition, exposure of a low-grade indolent tumor generated in the SV-HUC system. MC-T11, to the same carcinogens results in neoplastic progression as assessed by the production of high-grade aggressive cancers. In the present study, we show neoplastic progression of MC-T11 after in vitro exposure to a single dose of 6 Gy X rays. In addition, we show that the chromosome deletions, including losses of 4q, 11p, 13q and 18, observed in these radiation-induced tumors are similar to those observed in carcinogen-induced tumors, thus supporting the hypothesis that the experimental cell system, not the transforming agent, dictates the genetic losses required for tumorigenic transformation and progression.

Original languageEnglish (US)
Pages (from-to)86-92
Number of pages7
JournalRadiation Research
Volume138
Issue number1
StatePublished - 1994
Externally publishedYes

Fingerprint

carcinogens
progressions
grade
tumors
Radiation
neoplasms
Carcinogens
radiation
cancer
Neoplastic Cell Transformation
Neoplasms
cells
deletion
phenotype
bladder
chromosomes
viruses
Simian virus 40
chromosome elimination
Chromosome Deletion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Pazzaglia, S., Chen, X. R., Aamodt, C. B., Wu, S. Q., Kao, C., Gilchrist, K. W., ... Ritter, M. A. (1994). In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors. Radiation Research, 138(1), 86-92.

In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors. / Pazzaglia, S.; Chen, X. R.; Aamodt, C. B.; Wu, S. Q.; Kao, Chinghai; Gilchrist, K. W.; Oyasu, R.; Reznikoff, C. A.; Ritter, M. A.

In: Radiation Research, Vol. 138, No. 1, 1994, p. 86-92.

Research output: Contribution to journalArticle

Pazzaglia, S, Chen, XR, Aamodt, CB, Wu, SQ, Kao, C, Gilchrist, KW, Oyasu, R, Reznikoff, CA & Ritter, MA 1994, 'In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors', Radiation Research, vol. 138, no. 1, pp. 86-92.
Pazzaglia S, Chen XR, Aamodt CB, Wu SQ, Kao C, Gilchrist KW et al. In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors. Radiation Research. 1994;138(1):86-92.
Pazzaglia, S. ; Chen, X. R. ; Aamodt, C. B. ; Wu, S. Q. ; Kao, Chinghai ; Gilchrist, K. W. ; Oyasu, R. ; Reznikoff, C. A. ; Ritter, M. A. / In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors. In: Radiation Research. 1994 ; Vol. 138, No. 1. pp. 86-92.
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