In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors

S. Pazzaglia, X. R. Chen, C. B. Aamodt, S. Q. Wu, C. Kao, K. W. Gilchrist, R. Oyasu, C. A. Reznikoff, M. A. Ritter

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Abstract

Recent interest has focused on the identification of molecular genetic mechanisms in multistep neoplastic transformation. In vitro exposure of simian virus 40 (SV40)-immortalized human uroepithelial cells (SV-HUC) that are environmentally relevant to bladder carcinogens has been shown to produce tumorigenic transformation, as assessed by the ability of cells exposed to a carcinogen to form xenograph tumors with heterogeneous cancer phenotypes ranging from very aggressive, invasive high-grade carcinomas to superficial low-grade indolent tumors. In addition, exposure of a low-grade indolent tumor generated in the SV-HUC system. MC-T11, to the same carcinogens results in neoplastic progression as assessed by the production of high-grade aggressive cancers. In the present study, we show neoplastic progression of MC-T11 after in vitro exposure to a single dose of 6 Gy X rays. In addition, we show that the chromosome deletions, including losses of 4q, 11p, 13q and 18, observed in these radiation-induced tumors are similar to those observed in carcinogen-induced tumors, thus supporting the hypothesis that the experimental cell system, not the transforming agent, dictates the genetic losses required for tumorigenic transformation and progression.

Original languageEnglish (US)
Pages (from-to)86-92
Number of pages7
JournalRadiation research
Volume138
Issue number1
DOIs
StatePublished - Jan 1 1994

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ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Pazzaglia, S., Chen, X. R., Aamodt, C. B., Wu, S. Q., Kao, C., Gilchrist, K. W., Oyasu, R., Reznikoff, C. A., & Ritter, M. A. (1994). In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors. Radiation research, 138(1), 86-92. https://doi.org/10.2307/3578850