In vitro regulation of a mouse myelomonocytic leukemia line adapted to culture

Hal Broxmeyer, P. Ralph

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Regulation of the growth and production of granulocyte colony-stimulating activity (CSA) by WEHl-3 cells, a lysozyme-secreting mouse cell line adapted to culture, was investigated in vitro. WEHl-3 cloning efficiency is not enhanced by exogenously added CSA. However, WEHl-3 cloning efficiency in agar was suppressed by an activity in human polymorphonuclear neutrophil extract (colony-inhibiting activity) which inhibits endogenous WEHl-3 CSA production. The addition of increasing concentrations of WEHl-3 or L cell-conditioned medium containing CSA to CIA-depressed WEHl-3 agar cultures resulted in graded increases of cloning efficiency to that of the untreated sample. Testosterone, Deca-Durabolin, and bacterial lipopolysaccharide increased production, even when activating agents were added 1 day after the addition of colony-inhibiting activity. The activating agents had no colony-forming cells and did not enhance CSA activity. WEHl-3 cells responds to growth inhibitory and stimulatory activities and can serve as an in vitro model for studying the regulation of neoplastic cells.

Original languageEnglish (US)
Pages (from-to)3578-3584
Number of pages7
JournalCancer Research
Volume37
Issue number10
StatePublished - 1977
Externally publishedYes

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Leukemia
Organism Cloning
Agar
Muramidase
Conditioned Culture Medium
Growth
Granulocytes
Human Activities
Lipopolysaccharides
Testosterone
Neutrophils
In Vitro Techniques
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vitro regulation of a mouse myelomonocytic leukemia line adapted to culture. / Broxmeyer, Hal; Ralph, P.

In: Cancer Research, Vol. 37, No. 10, 1977, p. 3578-3584.

Research output: Contribution to journalArticle

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