In Vitro Regulation of a Mouse Myelomonocytic Leukemia Line Adapted to Culture

Hal E. Broxmeyer, Peter Ralph

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Regulation of the growth and production of granulocyte colony-stimulating activity (CSA) by WEHI-3 cells, a lysozyme-secreting mouse cell line adapted to culture, was investigated in vitro. WEHI-3 cloning efficiency is not enhanced by exogenously added CSA. However, WEHI-3 cloning efficiency in agar was suppressed by an activity in human polymorphonuclear neutrophil extract (colony-inhibiting activity) which inhibits endogenous WEHI-3 CSA production. The addition of increasing concentrations of WEHI-3-or L cell-conditioned medium containing CSA to CIA-depressed WEHI-3 agar cultures resulted in graded increases of cloning efficiency to that of the untreated sample. Testosterone, Deca-Durabolin, and bacterial lipopolysaccharide increased production of CSA by WEHI-3 cells and overcame colony-inhibiting activity-mediated suppression of CSA production, even when activating agents were added 1 day after the addition of colony-inhibiting activity. The activating agents had no direct stimulatory effect on normal mouse marrow colony-forming cells and did not enhance CSA activity. WEHI-3 cells respond to growth inhibitory and stimulatory activities and can serve as an in vitro model for studying the regulation of neoplastic cells.

Original languageEnglish (US)
Pages (from-to)3578-3584
Number of pages7
JournalCancer Research
Volume37
Issue number10
StatePublished - Oct 1977
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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