In vitro requirement for periostin in B lymphopoiesis

Basile T. Siewe, Susan L. Kalis, Phong T. Le, Pamela L. Witte, Sangdun Choi, Simon Conway, Laurel Druschitz, Katherine L. Knight

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

B lymphopoiesis arrests in rabbits by 4 months of age. To identify molecules that contribute to this arrest, cDNA-representational difference analysis on BM stromal cells from young and adult rabbits showed that expression of Postn that encodes for the extracellular matrix protein periostin dramatically reduced with age. Postn-small interfering RNA OP9 cells lost their capacity to support B-cell development from rabbit or murine BM cells, and reexpression of periostin restored this potential, indicating an in vitro requirement for periostin in B lymphopoiesis. In our system, we determined that periostin deficiency leads to increased cell death and decreased proliferation of B-lineage progenitors. Further, RGD peptide inhibition of periostin/αvβ3 interaction resulted in a marked decrease in B lymphopoiesis in vitro. Microarray analysis of the Postn-small interfering RNA OP9 cells showed decreased expression of key B-lymphopoietic factors, including IL-7 and CXCL12. In vivo, unidentified molecule(s) probably compensate periostin loss because Postn-/- mice had normal numbers of B-cell progenitors in BM. We conclude that the decline in periostin expression in adult rabbit BM does not solely explain the arrest of B lymphopoiesis. However, the interaction of periostin withαvβ3 on lymphoid progenitors probably provides both proliferative and survival signals for cells in the B-cell development pathway.

Original languageEnglish
Pages (from-to)3770-3779
Number of pages10
JournalBlood
Volume117
Issue number14
DOIs
StatePublished - Apr 7 2011

Fingerprint

Lymphopoiesis
Cells
Rabbits
Small Interfering RNA
Interleukin-7
Molecules
B-Lymphocytes
Extracellular Matrix Proteins
Cell death
Microarrays
B-Lymphoid Precursor Cells
Microarray Analysis
Stromal Cells
Complementary DNA
Young Adult
Cell Survival
Cell Death
In Vitro Techniques

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Siewe, B. T., Kalis, S. L., Le, P. T., Witte, P. L., Choi, S., Conway, S., ... Knight, K. L. (2011). In vitro requirement for periostin in B lymphopoiesis. Blood, 117(14), 3770-3779. https://doi.org/10.1182/blood-2010-08-301119

In vitro requirement for periostin in B lymphopoiesis. / Siewe, Basile T.; Kalis, Susan L.; Le, Phong T.; Witte, Pamela L.; Choi, Sangdun; Conway, Simon; Druschitz, Laurel; Knight, Katherine L.

In: Blood, Vol. 117, No. 14, 07.04.2011, p. 3770-3779.

Research output: Contribution to journalArticle

Siewe, BT, Kalis, SL, Le, PT, Witte, PL, Choi, S, Conway, S, Druschitz, L & Knight, KL 2011, 'In vitro requirement for periostin in B lymphopoiesis', Blood, vol. 117, no. 14, pp. 3770-3779. https://doi.org/10.1182/blood-2010-08-301119
Siewe BT, Kalis SL, Le PT, Witte PL, Choi S, Conway S et al. In vitro requirement for periostin in B lymphopoiesis. Blood. 2011 Apr 7;117(14):3770-3779. https://doi.org/10.1182/blood-2010-08-301119
Siewe, Basile T. ; Kalis, Susan L. ; Le, Phong T. ; Witte, Pamela L. ; Choi, Sangdun ; Conway, Simon ; Druschitz, Laurel ; Knight, Katherine L. / In vitro requirement for periostin in B lymphopoiesis. In: Blood. 2011 ; Vol. 117, No. 14. pp. 3770-3779.
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