In vivo characterization of vasocontractile activities in erythrocytes

M. P. McIlhany, L. M. Johns, T. Leipzig, N. J. Patronas, F. D. Brown, S. F. Mullan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Partially purified protein from washed and artificially hemolyzed erythrocytes, known to cause significant contractions of isolated canine cerebral vessels in vitro, was injected into the cisterna magna of intact anesthetized dogs. Cerebral blood flow, measured by the xenon-133 washout technique, decreased from a control value of 49.5 ± 1.17 ml/100 gm/min to an experimental value of 34.1 ± 1.65 ml/100 gm/min at 2 hours. Cerebral vascular resistance rose from a control value of 2.05 ± 0.17 PRU (peripheral resistance units) to an experimental value of 2.91 ± 0.25 PRU at 2 hours. Mean arterial blood pressure, heart rate, intracranial pressure, and cerebral perfusion pressure remained stable. Cardiac output also fell significantly (in 2-hour control animals it was 2.89 ± 0.37 liter/min, and in 2-hour experimental animals 1.43 ± 0.13 liter/min) and peripheral vascular resistance rose. These changes were evident by 10 minutes after the cisternal injection of the hemolysate protein, and remained for the duration of the 2-hour monitoring period. Serial vertebrobasilar angiograms demonstrated marked narrowing of the intracranial basilar artery when compared to control values. The narrowing persisted for several days in most animals, and tended to increaes with time. Relaxation occurred by the 10th through the 14th day. The authors conclude that this experimental preparation may be a useful model for both in vitro and in vivo investigation of chronic cerebral vasospasm.

Original languageEnglish (US)
Pages (from-to)356-361
Number of pages6
JournalJournal of Neurosurgery
Volume58
Issue number3
StatePublished - 1983
Externally publishedYes

Fingerprint

Vascular Resistance
Erythrocytes
Cerebrovascular Circulation
Arterial Pressure
Cisterna Magna
Intracranial Vasospasm
Xenon
Basilar Artery
Intracranial Pressure
Cardiac Output
Canidae
Angiography
Proteins
Heart Rate
Dogs
Injections
In Vitro Techniques

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

McIlhany, M. P., Johns, L. M., Leipzig, T., Patronas, N. J., Brown, F. D., & Mullan, S. F. (1983). In vivo characterization of vasocontractile activities in erythrocytes. Journal of Neurosurgery, 58(3), 356-361.

In vivo characterization of vasocontractile activities in erythrocytes. / McIlhany, M. P.; Johns, L. M.; Leipzig, T.; Patronas, N. J.; Brown, F. D.; Mullan, S. F.

In: Journal of Neurosurgery, Vol. 58, No. 3, 1983, p. 356-361.

Research output: Contribution to journalArticle

McIlhany, MP, Johns, LM, Leipzig, T, Patronas, NJ, Brown, FD & Mullan, SF 1983, 'In vivo characterization of vasocontractile activities in erythrocytes', Journal of Neurosurgery, vol. 58, no. 3, pp. 356-361.
McIlhany MP, Johns LM, Leipzig T, Patronas NJ, Brown FD, Mullan SF. In vivo characterization of vasocontractile activities in erythrocytes. Journal of Neurosurgery. 1983;58(3):356-361.
McIlhany, M. P. ; Johns, L. M. ; Leipzig, T. ; Patronas, N. J. ; Brown, F. D. ; Mullan, S. F. / In vivo characterization of vasocontractile activities in erythrocytes. In: Journal of Neurosurgery. 1983 ; Vol. 58, No. 3. pp. 356-361.
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