In vivo demonstration of a late depolarizing postsynaptic potential in CA1 pyramidal neurons

Yuan Fan, Bende Zou, Yiwen Ruan, Zhiping Pang, Zao C. Xu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Previous studies have shown that GABA can have a depolarizing and excitatory action through GABAA receptors in mature CNS neurons in vitro. However, it remains unknown whether this occurs under physiological conditions. In this study, using intracellular recording and staining in vivo technique, we show a late depolarizing postsynaptic potential (L-PSP) in CA1 pyramidal neurons of adult Wistar rats under halothane anesthesia. This L-PSP was elicited in ∼70% of the recorded neurons on stimulation of the Schaffer collaterals or the contralateral commissural path. The size of L-PSP was linearly correlated to the decay time constant but not the rising slope of the initial excitatory PSP (EPSP). Intravenous administration of the N-methyl-D-aspartate (NMDA) receptor blocker MK-801 and the GABAA receptor blocker picrotoxin significantly reduced the size of the L-PSP. The spine density and apical dendritic branching length of the neurons that displayed L-PSPs was significantly greater than those that do not. These results indicate that NMDA receptor and GABAA receptor-mediated depolarizing postsynaptic potentials can be revealed in CA1 pyramidal neurons of adult rats in vivo, supporting the physiological relevance of GABAA-mediated depolarization in normal neuronal information processing. The difference in electrophysiological properties and morphological features between neurons that display the L-PSP and the other neurons suggest that they might represent two different subtypes of CA1 pyramidal neurons.

Original languageEnglish
Pages (from-to)1326-1335
Number of pages10
JournalJournal of Neurophysiology
Volume93
Issue number3
DOIs
StatePublished - Mar 2005

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Synaptic Potentials
Pyramidal Cells
Neurons
GABA-A Receptors
N-Methyl-D-Aspartate Receptors
Picrotoxin
Dizocilpine Maleate
Halothane
Automatic Data Processing
Intravenous Administration
gamma-Aminobutyric Acid
Wistar Rats
Hippocampus
Spine
Anesthesia
Staining and Labeling

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

In vivo demonstration of a late depolarizing postsynaptic potential in CA1 pyramidal neurons. / Fan, Yuan; Zou, Bende; Ruan, Yiwen; Pang, Zhiping; Xu, Zao C.

In: Journal of Neurophysiology, Vol. 93, No. 3, 03.2005, p. 1326-1335.

Research output: Contribution to journalArticle

Fan, Yuan ; Zou, Bende ; Ruan, Yiwen ; Pang, Zhiping ; Xu, Zao C. / In vivo demonstration of a late depolarizing postsynaptic potential in CA1 pyramidal neurons. In: Journal of Neurophysiology. 2005 ; Vol. 93, No. 3. pp. 1326-1335.
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