In vivo effects of purified recombinant human macrophage colony- stimulating factor in combination with local hyperthermia on tumor progression in B16a melanoma bearing mice

L. Lu, M. Xiao, B. Wu, Xe Wang Wen Xe Wang, Ren Yan Wei Ren Yan, Hal Broxmeyer, R. N. Shen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Recombinant human (rhu) macrophage colony-stimulating factor (M-CSF) was evaluated, alone or in combination with local hyperthermia (LH), for their antitumor effects in mice inoculated with B16a melanoma cells. Several tumor related parameters and other hematopoietic and immunologic parameters were evaluated 5 weeks after subcutaneous (s.c.) inoculation of tumor cells into the right limbs of C57BL/6J male mice. RhuM-CSF was administered at 20 μg/injection, s.c., twice a day for 5 days/week for 2 weeks beginning 6 days after tumor cell inoculation and LH (43 ± 0.2°C) was given for 30 min twice/week for 2 weeks. Combined therapy prolonged survival of mice and caused significant inhibition of tumor growth, as measured by the volume or size of primary tumor, number and size of lung metastases, and chromatin fragment (CF) formation in tumor bearing mice, while treatment with M-CSF or LH alone had less or no effect. Combined therapy also resulted in increased numbers of splenic T-lymphocytes and the ratio of T-helper/suppressor cells, restoration of natural killer (NK) cell activity, increased numbers of peritoneal macrophages and their erythrophagocytosis capacity, and increased release or production of tumor necrosis factor (TNF)-α, but not interleukin (IL)-1α or IL-6. These results add to previous evidence that M-CSF might be a relevant therapeutic agent in combination with other therapies in the treatment of certain malignant diseases.

Original languageEnglish
Pages (from-to)139-152
Number of pages14
JournalInternational Journal of Hematology
Volume58
Issue number3
StatePublished - 1993

Fingerprint

Induced Hyperthermia
Macrophage Colony-Stimulating Factor
Melanoma
Neoplasms
Therapeutics
Peritoneal Macrophages
Subcutaneous Injections
Helper-Inducer T-Lymphocytes
Interleukin-1
Natural Killer Cells
Chromatin
Interleukin-6
Extremities
Tumor Necrosis Factor-alpha
Neoplasm Metastasis
T-Lymphocytes
Lung
Growth

ASJC Scopus subject areas

  • Hematology

Cite this

In vivo effects of purified recombinant human macrophage colony- stimulating factor in combination with local hyperthermia on tumor progression in B16a melanoma bearing mice. / Lu, L.; Xiao, M.; Wu, B.; Wen Xe Wang, Xe Wang; Wei Ren Yan, Ren Yan; Broxmeyer, Hal; Shen, R. N.

In: International Journal of Hematology, Vol. 58, No. 3, 1993, p. 139-152.

Research output: Contribution to journalArticle

@article{2354529dfef544b0bf90eba78a88a26b,
title = "In vivo effects of purified recombinant human macrophage colony- stimulating factor in combination with local hyperthermia on tumor progression in B16a melanoma bearing mice",
abstract = "Recombinant human (rhu) macrophage colony-stimulating factor (M-CSF) was evaluated, alone or in combination with local hyperthermia (LH), for their antitumor effects in mice inoculated with B16a melanoma cells. Several tumor related parameters and other hematopoietic and immunologic parameters were evaluated 5 weeks after subcutaneous (s.c.) inoculation of tumor cells into the right limbs of C57BL/6J male mice. RhuM-CSF was administered at 20 μg/injection, s.c., twice a day for 5 days/week for 2 weeks beginning 6 days after tumor cell inoculation and LH (43 ± 0.2°C) was given for 30 min twice/week for 2 weeks. Combined therapy prolonged survival of mice and caused significant inhibition of tumor growth, as measured by the volume or size of primary tumor, number and size of lung metastases, and chromatin fragment (CF) formation in tumor bearing mice, while treatment with M-CSF or LH alone had less or no effect. Combined therapy also resulted in increased numbers of splenic T-lymphocytes and the ratio of T-helper/suppressor cells, restoration of natural killer (NK) cell activity, increased numbers of peritoneal macrophages and their erythrophagocytosis capacity, and increased release or production of tumor necrosis factor (TNF)-α, but not interleukin (IL)-1α or IL-6. These results add to previous evidence that M-CSF might be a relevant therapeutic agent in combination with other therapies in the treatment of certain malignant diseases.",
author = "L. Lu and M. Xiao and B. Wu and {Wen Xe Wang}, {Xe Wang} and {Wei Ren Yan}, {Ren Yan} and Hal Broxmeyer and Shen, {R. N.}",
year = "1993",
language = "English",
volume = "58",
pages = "139--152",
journal = "International Journal of Hematology",
issn = "0925-5710",
publisher = "Springer Japan",
number = "3",

}

TY - JOUR

T1 - In vivo effects of purified recombinant human macrophage colony- stimulating factor in combination with local hyperthermia on tumor progression in B16a melanoma bearing mice

AU - Lu, L.

AU - Xiao, M.

AU - Wu, B.

AU - Wen Xe Wang, Xe Wang

AU - Wei Ren Yan, Ren Yan

AU - Broxmeyer, Hal

AU - Shen, R. N.

PY - 1993

Y1 - 1993

N2 - Recombinant human (rhu) macrophage colony-stimulating factor (M-CSF) was evaluated, alone or in combination with local hyperthermia (LH), for their antitumor effects in mice inoculated with B16a melanoma cells. Several tumor related parameters and other hematopoietic and immunologic parameters were evaluated 5 weeks after subcutaneous (s.c.) inoculation of tumor cells into the right limbs of C57BL/6J male mice. RhuM-CSF was administered at 20 μg/injection, s.c., twice a day for 5 days/week for 2 weeks beginning 6 days after tumor cell inoculation and LH (43 ± 0.2°C) was given for 30 min twice/week for 2 weeks. Combined therapy prolonged survival of mice and caused significant inhibition of tumor growth, as measured by the volume or size of primary tumor, number and size of lung metastases, and chromatin fragment (CF) formation in tumor bearing mice, while treatment with M-CSF or LH alone had less or no effect. Combined therapy also resulted in increased numbers of splenic T-lymphocytes and the ratio of T-helper/suppressor cells, restoration of natural killer (NK) cell activity, increased numbers of peritoneal macrophages and their erythrophagocytosis capacity, and increased release or production of tumor necrosis factor (TNF)-α, but not interleukin (IL)-1α or IL-6. These results add to previous evidence that M-CSF might be a relevant therapeutic agent in combination with other therapies in the treatment of certain malignant diseases.

AB - Recombinant human (rhu) macrophage colony-stimulating factor (M-CSF) was evaluated, alone or in combination with local hyperthermia (LH), for their antitumor effects in mice inoculated with B16a melanoma cells. Several tumor related parameters and other hematopoietic and immunologic parameters were evaluated 5 weeks after subcutaneous (s.c.) inoculation of tumor cells into the right limbs of C57BL/6J male mice. RhuM-CSF was administered at 20 μg/injection, s.c., twice a day for 5 days/week for 2 weeks beginning 6 days after tumor cell inoculation and LH (43 ± 0.2°C) was given for 30 min twice/week for 2 weeks. Combined therapy prolonged survival of mice and caused significant inhibition of tumor growth, as measured by the volume or size of primary tumor, number and size of lung metastases, and chromatin fragment (CF) formation in tumor bearing mice, while treatment with M-CSF or LH alone had less or no effect. Combined therapy also resulted in increased numbers of splenic T-lymphocytes and the ratio of T-helper/suppressor cells, restoration of natural killer (NK) cell activity, increased numbers of peritoneal macrophages and their erythrophagocytosis capacity, and increased release or production of tumor necrosis factor (TNF)-α, but not interleukin (IL)-1α or IL-6. These results add to previous evidence that M-CSF might be a relevant therapeutic agent in combination with other therapies in the treatment of certain malignant diseases.

UR - http://www.scopus.com/inward/record.url?scp=0027381764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027381764&partnerID=8YFLogxK

M3 - Article

C2 - 8148492

AN - SCOPUS:0027381764

VL - 58

SP - 139

EP - 152

JO - International Journal of Hematology

JF - International Journal of Hematology

SN - 0925-5710

IS - 3

ER -