In vivo expression of cell survival factors protein kinase C (PKC), nuclear factor κB (NFκB), and extracellular signal-regulated kinase (Erk), which may contribute to the development of radioresistance following radiotherapy, was looked for. Their modulation with natural compounds (curcumin, rutin or nicotinamide) was attempted in mice bearing a serially transplanted fibrosarcoma. Expression of protein kinase C was isoform specific. No translocation of any of the isozymes was noticed following γ-irradiation as has been reported elsewhere. None of the isoforms could be significantly inhibited by the modulators. However, significant inhibition of radiation-induced ERK and NFκB was observed with both curcumin and nicotinamide. Therefore we conclude that use of inhibitors of MAP kinases or NFκB may be a more promising strategy to enhance tumour cell killing or to prevent the development of radioresistance during radiotherapy.
- Radiation and fibrosarcoma
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Health, Toxicology and Mutagenesis