In vivo modulation of signaling factors involved in cell survival

Anirban Mitra, Malini Krishna

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In vivo expression of cell survival factors protein kinase C (PKC), nuclear factor κB (NFκB), and extracellular signal-regulated kinase (Erk), which may contribute to the development of radioresistance following radiotherapy, was looked for. Their modulation with natural compounds (curcumin, rutin or nicotinamide) was attempted in mice bearing a serially transplanted fibrosarcoma. Expression of protein kinase C was isoform specific. No translocation of any of the isozymes was noticed following γ-irradiation as has been reported elsewhere. None of the isoforms could be significantly inhibited by the modulators. However, significant inhibition of radiation-induced ERK and NFκB was observed with both curcumin and nicotinamide. Therefore we conclude that use of inhibitors of MAP kinases or NFκB may be a more promising strategy to enhance tumour cell killing or to prevent the development of radioresistance during radiotherapy.

Original languageEnglish (US)
Pages (from-to)491-495
Number of pages5
JournalJournal of Radiation Research
Volume45
Issue number4
DOIs
StatePublished - Dec 2004
Externally publishedYes

Fingerprint

Curcumin
Niacinamide
mitogen-activated protein kinase
Protein Kinase C
cell viability
Cell Survival
nicotinamide
radiation resistance
Protein Isoforms
curcumin
Radiotherapy
protein kinase C
radiotherapy
modulation
Rutin
Fibrosarcoma
Extracellular Signal-Regulated MAP Kinases
Isoenzymes
radiation therapy
fibrosarcoma

Keywords

  • ERK
  • NF-κB
  • PKC
  • Radiation and fibrosarcoma

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Radiation

Cite this

In vivo modulation of signaling factors involved in cell survival. / Mitra, Anirban; Krishna, Malini.

In: Journal of Radiation Research, Vol. 45, No. 4, 12.2004, p. 491-495.

Research output: Contribution to journalArticle

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