In vivo regulation of Bcl6 and T follicular helper cell development

Amanda C. Poholek, Kyle Hansen, Sairy G. Hernandez, Danelle Eto, Anmol Chandele, Jason S. Weinstein, Xuemei Dong, Jared M. Odegard, Susan M. Kaech, Alexander Dent, Shane Crotty, Joe Craft

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Follicular helper T (TFH) cells, defined by expression of the surface markers CXCR5 and programmed death receptor-1 (PD-1) and synthesis of IL-21, require upregulation of the transcriptional repressor Bcl6 for their development and function in B cell maturation in germinal centers. We have explored the role of B cells and the cytokines IL-6 and IL-21 in the in vivo regulation of Bcl6 expression and TFH cell development. We found that TFH cells are characterized by a Bcl6-dependent downregulation of P-selectin glycoprotein ligand 1 (PSGL1, a CCL19- and CCL21-binding protein), indicating that, like CXCR5 and PD-1 upregulation, modulation of PSGL1 expression is part of the TFH cell program of differentiation. B cells were neither required for initial upregulation of Bcl6 nor PSGL1 downregulation, suggesting these events preceded T-B cell interactions, although they were required for full development of the TFH cell phenotype, including CXCR5 and PD-1 upregulation, and IL-21 synthesis. Bcl6 upregulation and TFH cell differentiation were independent of IL-6 and IL-21, revealing that either cytokine is not absolutely required for development of Bcl6+ TFH cells in vivo. These data increase our understanding of Bcl6 regulation in TFH cells and their differentiation in vivo and identifies a new surface marker that may be functionally relevant in this subset.

Original languageEnglish
Pages (from-to)313-326
Number of pages14
JournalJournal of Immunology
Volume185
Issue number1
DOIs
StatePublished - Jul 1 2010

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Helper-Inducer T-Lymphocytes
Up-Regulation
Death Domain Receptors
B-Lymphocytes
Cell Differentiation
Interleukin-6
Down-Regulation
Cytokines
Germinal Center
Cell Communication
Carrier Proteins
Phenotype
interleukin-21

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Poholek, A. C., Hansen, K., Hernandez, S. G., Eto, D., Chandele, A., Weinstein, J. S., ... Craft, J. (2010). In vivo regulation of Bcl6 and T follicular helper cell development. Journal of Immunology, 185(1), 313-326. https://doi.org/10.4049/jimmunol.0904023

In vivo regulation of Bcl6 and T follicular helper cell development. / Poholek, Amanda C.; Hansen, Kyle; Hernandez, Sairy G.; Eto, Danelle; Chandele, Anmol; Weinstein, Jason S.; Dong, Xuemei; Odegard, Jared M.; Kaech, Susan M.; Dent, Alexander; Crotty, Shane; Craft, Joe.

In: Journal of Immunology, Vol. 185, No. 1, 01.07.2010, p. 313-326.

Research output: Contribution to journalArticle

Poholek, AC, Hansen, K, Hernandez, SG, Eto, D, Chandele, A, Weinstein, JS, Dong, X, Odegard, JM, Kaech, SM, Dent, A, Crotty, S & Craft, J 2010, 'In vivo regulation of Bcl6 and T follicular helper cell development', Journal of Immunology, vol. 185, no. 1, pp. 313-326. https://doi.org/10.4049/jimmunol.0904023
Poholek AC, Hansen K, Hernandez SG, Eto D, Chandele A, Weinstein JS et al. In vivo regulation of Bcl6 and T follicular helper cell development. Journal of Immunology. 2010 Jul 1;185(1):313-326. https://doi.org/10.4049/jimmunol.0904023
Poholek, Amanda C. ; Hansen, Kyle ; Hernandez, Sairy G. ; Eto, Danelle ; Chandele, Anmol ; Weinstein, Jason S. ; Dong, Xuemei ; Odegard, Jared M. ; Kaech, Susan M. ; Dent, Alexander ; Crotty, Shane ; Craft, Joe. / In vivo regulation of Bcl6 and T follicular helper cell development. In: Journal of Immunology. 2010 ; Vol. 185, No. 1. pp. 313-326.
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