In vivo tissue repair using light-activated surgical adhesive in a porcine model

K. M. McNally-Heintzelman, J. N. Riley, T. J. Dickson, D. M. Hou, P. Rogers, K. L. March

Research output: Chapter in Book/Report/Conference proceedingConference contribution

9 Citations (Scopus)

Abstract

An in vivo study was conducted to investigate the feasibility, mechanical function, and chronic biocompatibility of a new light-activated surgical adhesive for achieving rapid hemostasis of the puncture site following diagnostic catheterization and interventional cardiac procedures. Porcine carotid arteries (n=6) and femoral arteries (n=6) were exposed, and an incision was made in the arterial walls using a 16G needle. The surgical adhesive, composed of a poly(L-lactic-co-glycolic acid) scaffold doped with the traditional protein solder mix of serum albumin and indocyanine green dye, was used to close the incisions in conjunction with an 805nm diode laser. Blood flow was restored to the vessels immediately after the procedure and the incision sites were checked for patency. The strength and hemostatic abilities of the new surgical adhesive were evaluated in the context of arterial pressure, persistence of hemostasis and presence of any inflammatory reaction after 3 days. After this evaluation period, the surgical procedure was repeated on the carotid arteries (n=6) and femoral arteries (n=6) of three additional animals that had been heparinized prior to surgery to closer approximate the conditions seen in a typical vascular surgical setting. The patency rate of both the unheparinized and heparinized vessels was 100% at 3 days post-operative with evidence of intraluminal thrombosis seen in only one of the repaired vessels. The adhesive technique also compared favorably with a previous study conducted using conventional suture techniques. Repairs formed with the adhesive technique were achieved more rapidly than suturing, and acute leakage was observed less frequently. The new light-activated surgical adhesive thus has great promise as an alternative to conventional repair techniques for achieving rapid vascular hemostasis following cardiac procedures.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsR.R. Anderson, K.E. Bartels, L.S. Bass, C.G. Garrett
Pages226-232
Number of pages7
Volume4244
DOIs
StatePublished - 2001
EventLaser in Surgery: Advanced Characterization, Therapeutics, and Systems XI - San Jose,CA, United States
Duration: Jun 20 2001Jun 23 2001

Other

OtherLaser in Surgery: Advanced Characterization, Therapeutics, and Systems XI
CountryUnited States
CitySan Jose,CA
Period6/20/016/23/01

Fingerprint

hemostatics
adhesives
Adhesives
Repair
Tissue
arteries
vessels
catheterization
thrombosis
biocompatibility
Scaffolds (biology)
blood flow
solders
albumins
Biocompatibility
surgery
needles
Needles
serums
Soldering alloys

Keywords

  • Albumin protein solder
  • Diode laser
  • Hemostasis
  • Indocyanine green dye
  • Polymer membrane
  • Puncture site

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

McNally-Heintzelman, K. M., Riley, J. N., Dickson, T. J., Hou, D. M., Rogers, P., & March, K. L. (2001). In vivo tissue repair using light-activated surgical adhesive in a porcine model. In R. R. Anderson, K. E. Bartels, L. S. Bass, & C. G. Garrett (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4244, pp. 226-232) https://doi.org/10.1117/12.427796

In vivo tissue repair using light-activated surgical adhesive in a porcine model. / McNally-Heintzelman, K. M.; Riley, J. N.; Dickson, T. J.; Hou, D. M.; Rogers, P.; March, K. L.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / R.R. Anderson; K.E. Bartels; L.S. Bass; C.G. Garrett. Vol. 4244 2001. p. 226-232.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

McNally-Heintzelman, KM, Riley, JN, Dickson, TJ, Hou, DM, Rogers, P & March, KL 2001, In vivo tissue repair using light-activated surgical adhesive in a porcine model. in RR Anderson, KE Bartels, LS Bass & CG Garrett (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4244, pp. 226-232, Laser in Surgery: Advanced Characterization, Therapeutics, and Systems XI, San Jose,CA, United States, 6/20/01. https://doi.org/10.1117/12.427796
McNally-Heintzelman KM, Riley JN, Dickson TJ, Hou DM, Rogers P, March KL. In vivo tissue repair using light-activated surgical adhesive in a porcine model. In Anderson RR, Bartels KE, Bass LS, Garrett CG, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4244. 2001. p. 226-232 https://doi.org/10.1117/12.427796
McNally-Heintzelman, K. M. ; Riley, J. N. ; Dickson, T. J. ; Hou, D. M. ; Rogers, P. ; March, K. L. / In vivo tissue repair using light-activated surgical adhesive in a porcine model. Proceedings of SPIE - The International Society for Optical Engineering. editor / R.R. Anderson ; K.E. Bartels ; L.S. Bass ; C.G. Garrett. Vol. 4244 2001. pp. 226-232
@inproceedings{52f6883904554c8aad3e0c9074c428b4,
title = "In vivo tissue repair using light-activated surgical adhesive in a porcine model",
abstract = "An in vivo study was conducted to investigate the feasibility, mechanical function, and chronic biocompatibility of a new light-activated surgical adhesive for achieving rapid hemostasis of the puncture site following diagnostic catheterization and interventional cardiac procedures. Porcine carotid arteries (n=6) and femoral arteries (n=6) were exposed, and an incision was made in the arterial walls using a 16G needle. The surgical adhesive, composed of a poly(L-lactic-co-glycolic acid) scaffold doped with the traditional protein solder mix of serum albumin and indocyanine green dye, was used to close the incisions in conjunction with an 805nm diode laser. Blood flow was restored to the vessels immediately after the procedure and the incision sites were checked for patency. The strength and hemostatic abilities of the new surgical adhesive were evaluated in the context of arterial pressure, persistence of hemostasis and presence of any inflammatory reaction after 3 days. After this evaluation period, the surgical procedure was repeated on the carotid arteries (n=6) and femoral arteries (n=6) of three additional animals that had been heparinized prior to surgery to closer approximate the conditions seen in a typical vascular surgical setting. The patency rate of both the unheparinized and heparinized vessels was 100{\%} at 3 days post-operative with evidence of intraluminal thrombosis seen in only one of the repaired vessels. The adhesive technique also compared favorably with a previous study conducted using conventional suture techniques. Repairs formed with the adhesive technique were achieved more rapidly than suturing, and acute leakage was observed less frequently. The new light-activated surgical adhesive thus has great promise as an alternative to conventional repair techniques for achieving rapid vascular hemostasis following cardiac procedures.",
keywords = "Albumin protein solder, Diode laser, Hemostasis, Indocyanine green dye, Polymer membrane, Puncture site",
author = "McNally-Heintzelman, {K. M.} and Riley, {J. N.} and Dickson, {T. J.} and Hou, {D. M.} and P. Rogers and March, {K. L.}",
year = "2001",
doi = "10.1117/12.427796",
language = "English (US)",
volume = "4244",
pages = "226--232",
editor = "R.R. Anderson and K.E. Bartels and L.S. Bass and C.G. Garrett",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - In vivo tissue repair using light-activated surgical adhesive in a porcine model

AU - McNally-Heintzelman, K. M.

AU - Riley, J. N.

AU - Dickson, T. J.

AU - Hou, D. M.

AU - Rogers, P.

AU - March, K. L.

PY - 2001

Y1 - 2001

N2 - An in vivo study was conducted to investigate the feasibility, mechanical function, and chronic biocompatibility of a new light-activated surgical adhesive for achieving rapid hemostasis of the puncture site following diagnostic catheterization and interventional cardiac procedures. Porcine carotid arteries (n=6) and femoral arteries (n=6) were exposed, and an incision was made in the arterial walls using a 16G needle. The surgical adhesive, composed of a poly(L-lactic-co-glycolic acid) scaffold doped with the traditional protein solder mix of serum albumin and indocyanine green dye, was used to close the incisions in conjunction with an 805nm diode laser. Blood flow was restored to the vessels immediately after the procedure and the incision sites were checked for patency. The strength and hemostatic abilities of the new surgical adhesive were evaluated in the context of arterial pressure, persistence of hemostasis and presence of any inflammatory reaction after 3 days. After this evaluation period, the surgical procedure was repeated on the carotid arteries (n=6) and femoral arteries (n=6) of three additional animals that had been heparinized prior to surgery to closer approximate the conditions seen in a typical vascular surgical setting. The patency rate of both the unheparinized and heparinized vessels was 100% at 3 days post-operative with evidence of intraluminal thrombosis seen in only one of the repaired vessels. The adhesive technique also compared favorably with a previous study conducted using conventional suture techniques. Repairs formed with the adhesive technique were achieved more rapidly than suturing, and acute leakage was observed less frequently. The new light-activated surgical adhesive thus has great promise as an alternative to conventional repair techniques for achieving rapid vascular hemostasis following cardiac procedures.

AB - An in vivo study was conducted to investigate the feasibility, mechanical function, and chronic biocompatibility of a new light-activated surgical adhesive for achieving rapid hemostasis of the puncture site following diagnostic catheterization and interventional cardiac procedures. Porcine carotid arteries (n=6) and femoral arteries (n=6) were exposed, and an incision was made in the arterial walls using a 16G needle. The surgical adhesive, composed of a poly(L-lactic-co-glycolic acid) scaffold doped with the traditional protein solder mix of serum albumin and indocyanine green dye, was used to close the incisions in conjunction with an 805nm diode laser. Blood flow was restored to the vessels immediately after the procedure and the incision sites were checked for patency. The strength and hemostatic abilities of the new surgical adhesive were evaluated in the context of arterial pressure, persistence of hemostasis and presence of any inflammatory reaction after 3 days. After this evaluation period, the surgical procedure was repeated on the carotid arteries (n=6) and femoral arteries (n=6) of three additional animals that had been heparinized prior to surgery to closer approximate the conditions seen in a typical vascular surgical setting. The patency rate of both the unheparinized and heparinized vessels was 100% at 3 days post-operative with evidence of intraluminal thrombosis seen in only one of the repaired vessels. The adhesive technique also compared favorably with a previous study conducted using conventional suture techniques. Repairs formed with the adhesive technique were achieved more rapidly than suturing, and acute leakage was observed less frequently. The new light-activated surgical adhesive thus has great promise as an alternative to conventional repair techniques for achieving rapid vascular hemostasis following cardiac procedures.

KW - Albumin protein solder

KW - Diode laser

KW - Hemostasis

KW - Indocyanine green dye

KW - Polymer membrane

KW - Puncture site

UR - http://www.scopus.com/inward/record.url?scp=0034931650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034931650&partnerID=8YFLogxK

U2 - 10.1117/12.427796

DO - 10.1117/12.427796

M3 - Conference contribution

AN - SCOPUS:0034931650

VL - 4244

SP - 226

EP - 232

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Anderson, R.R.

A2 - Bartels, K.E.

A2 - Bass, L.S.

A2 - Garrett, C.G.

ER -