Inactivation of the haemophilus ducreyi luxS gene affects the virulence of this pathogen in human subjects

Maria Labandeira-Rey, Diane Janowicz, Robert J. Blick, Kate R. Fortney, Beth Zwickl, Barry Katz, Stanley Spinola, Eric J. Hansen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Haemophilus ducreyi 35000HP contains a homologue of the luxS gene, which encodes an enzyme that synthesizes autoinducer 2 (AI-2) in other gram-negative bacteria. H. ducreyi 35000HP produced AI-2 that functioned in a Vibrio harveyi-based reporter system. A H. ducreyi luxS mutant was constructed by insertional inactivation of the luxS gene and lost the ability to produce AI-2. Provision of the H. ducreyi luxS gene in trans partially restored AI-2 production by the mutant. The luxS mutant was compared with its parent for virulence in the human challenge model of experimental chancroid. The pustule-formation rate in 5 volunteers was 93.3% (95% confidence interval, 81.7%-99.9%) at 15 parent sites and 60.0% (95% confidence interval, 48.3%-71.7%) at 15 mutant sites (1-tailed P<.001). Thus, the luxS mutant was partially attenuated for virulence. This is the first report of AI-2 production contributing to the pathogenesis of a genital ulcer disease.

Original languageEnglish
Pages (from-to)409-416
Number of pages8
JournalJournal of Infectious Diseases
Volume200
Issue number3
DOIs
StatePublished - Aug 1 2009

Fingerprint

Haemophilus ducreyi
Virulence
Genes
Chancroid
Confidence Intervals
Vibrio
Gene Silencing
Gram-Negative Bacteria
Ulcer
Volunteers
Theoretical Models
N-octanoylhomoserine lactone
Enzymes

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Inactivation of the haemophilus ducreyi luxS gene affects the virulence of this pathogen in human subjects. / Labandeira-Rey, Maria; Janowicz, Diane; Blick, Robert J.; Fortney, Kate R.; Zwickl, Beth; Katz, Barry; Spinola, Stanley; Hansen, Eric J.

In: Journal of Infectious Diseases, Vol. 200, No. 3, 01.08.2009, p. 409-416.

Research output: Contribution to journalArticle

Labandeira-Rey, Maria ; Janowicz, Diane ; Blick, Robert J. ; Fortney, Kate R. ; Zwickl, Beth ; Katz, Barry ; Spinola, Stanley ; Hansen, Eric J. / Inactivation of the haemophilus ducreyi luxS gene affects the virulence of this pathogen in human subjects. In: Journal of Infectious Diseases. 2009 ; Vol. 200, No. 3. pp. 409-416.
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