Increased class Ib antigen display on TAP-2 mutant cells by a mitochondrial function inhibitor

Evan Hermel, Elena Grigorenko, Carla J. Aldrich

Research output: Contribution to journalArticle

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Abstract

Class I histocompatibility antigen display is defective in the RMA-S mutant cell line due to a mutation in the Tap-2 gone, which encodes a peptide transporter. Incubation of RMA-S cells with oligomycin, an inhibitor of mitochondrial ATPase, strongly increased lysis by cytotoxic T lymphocytes (CTL) specific for the class Ib antigen H2-M3, and lysis by Qa-1b-specific CTL was restored. Oligomycin did not affect normal class I display on RMA cells. Treatment of RMA-S cells with other inhibitors of mitochondrial function failed to increase lysis by anti-H2-M3 or Qa-1b CTL. Lysis by allogenic CTL specific for H-2b antigens was either not enhanced or only weakly increased, depending upon the H-2 haplotype of the alloreactive effector cells used.

Original languageEnglish (US)
Pages (from-to)10-15
Number of pages6
JournalCellular Immunology
Volume179
Issue number1
DOIs
StatePublished - Jul 10 1997

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Cytotoxic T-Lymphocytes
Oligomycins
Antigens
Histocompatibility Antigens Class I
Haplotypes
Adenosine Triphosphatases
Cell Line
Mutation

ASJC Scopus subject areas

  • Immunology

Cite this

Increased class Ib antigen display on TAP-2 mutant cells by a mitochondrial function inhibitor. / Hermel, Evan; Grigorenko, Elena; Aldrich, Carla J.

In: Cellular Immunology, Vol. 179, No. 1, 10.07.1997, p. 10-15.

Research output: Contribution to journalArticle

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