Increased cyclin D1 expression in chronic pancreatitis

Marko Kornmann, Toshiyuki Ishiwata, Nadir Arber, Hans G. Beger, Murray Korc

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cyclin D1 is an important regulator of the G1 phase of the cell cycle. In this study we examined the expression and localization of cyclin D1 in chronic pancreatitis (CP), a condition associated with fibrosis, acinar cell degeneration, and foci of duct cell proliferation. Northern blotting of total RNA from the normal pancreas and from the pancreas of patients with CP revealed the presence of 4.4-kb cyclin D1 mRNA transcript in all samples. Densitometric analysis indicated that pancreatic cyclin D1 mRNA levels were 2.0-fold increased in CP compared to normal controls (p = 0.0046). In the normal pancreas, faint cyclin D1 immunoreactivity was present in the nuclei and cytoplasm of some ductal cells and islet cell. In contrast, in CO, moderate to strong cyclin D1 immunoreactivity was present in many ductal and islet cells, as well as in some acinar cells and fibroblasts. These findings rase the possibility that enhanced cyclin D1 expression may contribute to a hyperproliferative state in CP.

Original languageEnglish (US)
Pages (from-to)158-162
Number of pages5
JournalPancreas
Volume17
Issue number2
StatePublished - Aug 1998
Externally publishedYes

Fingerprint

Cyclin D1
Chronic Pancreatitis
Pancreas
Acinar Cells
Islets of Langerhans
Messenger RNA
G1 Phase
Carbon Monoxide
Northern Blotting
Cell Cycle
Cytoplasm
Fibrosis
Fibroblasts
Cell Proliferation
RNA

Keywords

  • Chronic pancreatitis
  • Cyclin D1

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology

Cite this

Kornmann, M., Ishiwata, T., Arber, N., Beger, H. G., & Korc, M. (1998). Increased cyclin D1 expression in chronic pancreatitis. Pancreas, 17(2), 158-162.

Increased cyclin D1 expression in chronic pancreatitis. / Kornmann, Marko; Ishiwata, Toshiyuki; Arber, Nadir; Beger, Hans G.; Korc, Murray.

In: Pancreas, Vol. 17, No. 2, 08.1998, p. 158-162.

Research output: Contribution to journalArticle

Kornmann, M, Ishiwata, T, Arber, N, Beger, HG & Korc, M 1998, 'Increased cyclin D1 expression in chronic pancreatitis', Pancreas, vol. 17, no. 2, pp. 158-162.
Kornmann M, Ishiwata T, Arber N, Beger HG, Korc M. Increased cyclin D1 expression in chronic pancreatitis. Pancreas. 1998 Aug;17(2):158-162.
Kornmann, Marko ; Ishiwata, Toshiyuki ; Arber, Nadir ; Beger, Hans G. ; Korc, Murray. / Increased cyclin D1 expression in chronic pancreatitis. In: Pancreas. 1998 ; Vol. 17, No. 2. pp. 158-162.
@article{6eb6115b90a94a5db41b9c991c59f830,
title = "Increased cyclin D1 expression in chronic pancreatitis",
abstract = "Cyclin D1 is an important regulator of the G1 phase of the cell cycle. In this study we examined the expression and localization of cyclin D1 in chronic pancreatitis (CP), a condition associated with fibrosis, acinar cell degeneration, and foci of duct cell proliferation. Northern blotting of total RNA from the normal pancreas and from the pancreas of patients with CP revealed the presence of 4.4-kb cyclin D1 mRNA transcript in all samples. Densitometric analysis indicated that pancreatic cyclin D1 mRNA levels were 2.0-fold increased in CP compared to normal controls (p = 0.0046). In the normal pancreas, faint cyclin D1 immunoreactivity was present in the nuclei and cytoplasm of some ductal cells and islet cell. In contrast, in CO, moderate to strong cyclin D1 immunoreactivity was present in many ductal and islet cells, as well as in some acinar cells and fibroblasts. These findings rase the possibility that enhanced cyclin D1 expression may contribute to a hyperproliferative state in CP.",
keywords = "Chronic pancreatitis, Cyclin D1",
author = "Marko Kornmann and Toshiyuki Ishiwata and Nadir Arber and Beger, {Hans G.} and Murray Korc",
year = "1998",
month = "8",
language = "English (US)",
volume = "17",
pages = "158--162",
journal = "Pancreas",
issn = "0885-3177",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Increased cyclin D1 expression in chronic pancreatitis

AU - Kornmann, Marko

AU - Ishiwata, Toshiyuki

AU - Arber, Nadir

AU - Beger, Hans G.

AU - Korc, Murray

PY - 1998/8

Y1 - 1998/8

N2 - Cyclin D1 is an important regulator of the G1 phase of the cell cycle. In this study we examined the expression and localization of cyclin D1 in chronic pancreatitis (CP), a condition associated with fibrosis, acinar cell degeneration, and foci of duct cell proliferation. Northern blotting of total RNA from the normal pancreas and from the pancreas of patients with CP revealed the presence of 4.4-kb cyclin D1 mRNA transcript in all samples. Densitometric analysis indicated that pancreatic cyclin D1 mRNA levels were 2.0-fold increased in CP compared to normal controls (p = 0.0046). In the normal pancreas, faint cyclin D1 immunoreactivity was present in the nuclei and cytoplasm of some ductal cells and islet cell. In contrast, in CO, moderate to strong cyclin D1 immunoreactivity was present in many ductal and islet cells, as well as in some acinar cells and fibroblasts. These findings rase the possibility that enhanced cyclin D1 expression may contribute to a hyperproliferative state in CP.

AB - Cyclin D1 is an important regulator of the G1 phase of the cell cycle. In this study we examined the expression and localization of cyclin D1 in chronic pancreatitis (CP), a condition associated with fibrosis, acinar cell degeneration, and foci of duct cell proliferation. Northern blotting of total RNA from the normal pancreas and from the pancreas of patients with CP revealed the presence of 4.4-kb cyclin D1 mRNA transcript in all samples. Densitometric analysis indicated that pancreatic cyclin D1 mRNA levels were 2.0-fold increased in CP compared to normal controls (p = 0.0046). In the normal pancreas, faint cyclin D1 immunoreactivity was present in the nuclei and cytoplasm of some ductal cells and islet cell. In contrast, in CO, moderate to strong cyclin D1 immunoreactivity was present in many ductal and islet cells, as well as in some acinar cells and fibroblasts. These findings rase the possibility that enhanced cyclin D1 expression may contribute to a hyperproliferative state in CP.

KW - Chronic pancreatitis

KW - Cyclin D1

UR - http://www.scopus.com/inward/record.url?scp=0031868407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031868407&partnerID=8YFLogxK

M3 - Article

C2 - 9700947

AN - SCOPUS:0031868407

VL - 17

SP - 158

EP - 162

JO - Pancreas

JF - Pancreas

SN - 0885-3177

IS - 2

ER -