Increased expression of acidic and basic fibroblast growth factors in chronic pancreatitis

Helmut Friess, Yoichiro Yamanaka, Markus Büchler, Hans G. Beger, David A. Do, Michael S. Kobrin, Murray Korc

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) belong to a family of mitogenic polypeptides that are involved in cellular proliferation and differentiation. In this study we investigated the potential role of aFGF and bFGF in chronic pancreatitis (CP), a fibrotic condition associated with acinar cell dedifferentiation and atrophy, and fibroblastic proliferation. By immunohistochemistry, aFGF and bFGF were abundant in pancreatic ductal and acinar cells in pancreatic tissues from CP patients. Immunoblotting with the same highly specific monoclonal antibodies demonstrated a marked increase in aFGF and bFGF in pancreatic homogenates from CP patients by comparison with the normal pancreas. Northern blot analysis indicated that, by comparison with normal controls, 16 of 21 CP tissues exhibited a 14-fold increase in aFGF mRNA levels, and 19 of 21 CP tissues exhibited a 15-fold increase in bFGF mRNA levels. In situ hybridization confirmed that this overexpression occurred in ductal and acinar cells, and indicated that both mRNA moieties colocalized with their respective proteins. These findings suggest that aFGF and bFGF may either be involved in the pathobiological mechanisms that occur in CP, or that their overexpression may be the consequence of other perturbations that occur in this disorder.

Original languageEnglish (US)
Pages (from-to)117-128
Number of pages12
JournalAmerican Journal of Pathology
Volume144
Issue number1
StatePublished - Jan 1994
Externally publishedYes

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Fibroblast Growth Factor 1
Chronic Pancreatitis
Fibroblast Growth Factor 2
Acinar Cells
Messenger RNA
Cell Dedifferentiation
Immunoblotting
Northern Blotting
Atrophy
In Situ Hybridization
Pancreas
Immunohistochemistry
Monoclonal Antibodies
Cell Proliferation
Peptides
Proteins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Friess, H., Yamanaka, Y., Büchler, M., Beger, H. G., Do, D. A., Kobrin, M. S., & Korc, M. (1994). Increased expression of acidic and basic fibroblast growth factors in chronic pancreatitis. American Journal of Pathology, 144(1), 117-128.

Increased expression of acidic and basic fibroblast growth factors in chronic pancreatitis. / Friess, Helmut; Yamanaka, Yoichiro; Büchler, Markus; Beger, Hans G.; Do, David A.; Kobrin, Michael S.; Korc, Murray.

In: American Journal of Pathology, Vol. 144, No. 1, 01.1994, p. 117-128.

Research output: Contribution to journalArticle

Friess, H, Yamanaka, Y, Büchler, M, Beger, HG, Do, DA, Kobrin, MS & Korc, M 1994, 'Increased expression of acidic and basic fibroblast growth factors in chronic pancreatitis', American Journal of Pathology, vol. 144, no. 1, pp. 117-128.
Friess H, Yamanaka Y, Büchler M, Beger HG, Do DA, Kobrin MS et al. Increased expression of acidic and basic fibroblast growth factors in chronic pancreatitis. American Journal of Pathology. 1994 Jan;144(1):117-128.
Friess, Helmut ; Yamanaka, Yoichiro ; Büchler, Markus ; Beger, Hans G. ; Do, David A. ; Kobrin, Michael S. ; Korc, Murray. / Increased expression of acidic and basic fibroblast growth factors in chronic pancreatitis. In: American Journal of Pathology. 1994 ; Vol. 144, No. 1. pp. 117-128.
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