Increased expression of chemokines in the skin of chronic proliferative dermatitis mutant mice

Matthew L. Renninger, Rosemarie Seymour, James W. Lillard, John P. Sundberg, Harm HogenEsch

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chemokines direct the migration of leukocytes to sites of inflammation and are potential targets for anti-inflammatory therapy. Chronic proliferative dermatitis (cpdm/cpdm) mutant mice develop a persistent eosinophilic dermatitis associated with increased TH2 cytokines in the skin. Expression patterns of chemokines in the skin of cpdm/cpdm mice were evaluated to define the mechanisms driving cutaneous infiltration by leukocytes. RNA isolated from the skin of mutant and littermate control mice revealed a significant increase in Ccl1 (TCA-3), Ccl2 (MCP-1), Ccl11 (eotaxin), Ccl17 (TARC), Cxcl10 (IP-10), and the chemokine receptor Ccr3. The concentration of CCL11 protein was increased two- to threefold in the skin of cpdm/cpdm mice by enzyme-linked immunosorbent assay. In vitro culture of primary dermal fibroblasts from cpdm/cpdm and control mice with tumor necrosis factor, IL-4, and IL-13 stimulation did not reveal differences in their ability to secrete CCL11, suggesting that the increased chemokine expression observed in the skin of cpdm/cpdm mice is most likely caused by the increased TH2 cytokines in the dermis of this mouse model. Treatment of cpdm/cpdm mice with CCL11-neutralizing polyclonal antibodies did not affect the number of eosinophils in the skin or the severity of the dermatitis. Neutralizing multiple chemokines or chemokine receptors may be necessary to decrease eosinophil accumulation. The cpdm/cpdm mutant mouse is a potentially useful model to determine the role of various chemokines in eosinophil accumulation in chronic inflammation.

Original languageEnglish (US)
Pages (from-to)906-913
Number of pages8
JournalExperimental Dermatology
Volume14
Issue number12
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Dermatitis
Chemokines
Skin
Chemokine Receptors
Eosinophils
Cytokines
Immunosorbents
Interleukin-13
Chemokine CCL11
Leukocytes
Fibroblasts
Cell culture
Infiltration
Interleukin-4
Inflammation
Assays
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
RNA
Dermis

Keywords

  • Chemokines
  • Dermatitis
  • Eosinophils
  • Mouse

ASJC Scopus subject areas

  • Dermatology

Cite this

Increased expression of chemokines in the skin of chronic proliferative dermatitis mutant mice. / Renninger, Matthew L.; Seymour, Rosemarie; Lillard, James W.; Sundberg, John P.; HogenEsch, Harm.

In: Experimental Dermatology, Vol. 14, No. 12, 12.2005, p. 906-913.

Research output: Contribution to journalArticle

Renninger, Matthew L. ; Seymour, Rosemarie ; Lillard, James W. ; Sundberg, John P. ; HogenEsch, Harm. / Increased expression of chemokines in the skin of chronic proliferative dermatitis mutant mice. In: Experimental Dermatology. 2005 ; Vol. 14, No. 12. pp. 906-913.
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