Increased Expression of Keratinocyte Growth Factor in Human Pancreatic Cancer

I. Siddiqi, H. Funatomi, M. S. Kobrin, H. Friess, M. W. Buchler, Murray Korc

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Keratinocyte growth factor (KGF) is a member of the fibroblast growth factor (FGF) group of heparin-binding polypeptides. In the present study we sought to determine whether KGF is expressed in human pancreatic cancers. Using reverse transcriptase polymerase chain reaction (RT-PCR), a cDNA fragment of KGF was cloned and used to analyze Northern blots of RNA isolated from normal and cancerous human pancreatic tissues. Seven of 16 (44%) pancreatic cancer samples revealed significant overexpression of the 2.4 kilobase KGF mRNA transcript by comparison with the normal pancreas. Northern blot analysis failed to reveal the KGF transcript in several cultured human pancreatic cancer cell lines. However, by PCR analysis, some of the cell lines expressed KGF mRNA. Furthermore, 5 of 7 tested cell lines expressed the KGF receptor, and the growth of one cell line was enhanced by human recombinant KGF. These results suggest that KGF may participate in aberrant paracrine and autocrine pathways in human pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume215
Issue number1
DOIs
StatePublished - Oct 4 1995
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 7
Pancreatic Neoplasms
Cells
Cell Line
Northern Blotting
Messenger RNA
Fibroblast Growth Factors
human FGF7 protein
Reverse Transcriptase Polymerase Chain Reaction
Polymerase chain reaction
RNA-Directed DNA Polymerase
Heparin
Pancreas
Complementary DNA
RNA
Polymerase Chain Reaction
Peptides
Tissue
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Biophysics
  • Biochemistry

Cite this

Increased Expression of Keratinocyte Growth Factor in Human Pancreatic Cancer. / Siddiqi, I.; Funatomi, H.; Kobrin, M. S.; Friess, H.; Buchler, M. W.; Korc, Murray.

In: Biochemical and Biophysical Research Communications, Vol. 215, No. 1, 04.10.1995, p. 309-315.

Research output: Contribution to journalArticle

Siddiqi, I, Funatomi, H, Kobrin, MS, Friess, H, Buchler, MW & Korc, M 1995, 'Increased Expression of Keratinocyte Growth Factor in Human Pancreatic Cancer', Biochemical and Biophysical Research Communications, vol. 215, no. 1, pp. 309-315. https://doi.org/10.1006/bbrc.1995.2467
Siddiqi, I. ; Funatomi, H. ; Kobrin, M. S. ; Friess, H. ; Buchler, M. W. ; Korc, Murray. / Increased Expression of Keratinocyte Growth Factor in Human Pancreatic Cancer. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 215, No. 1. pp. 309-315.
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