Increased expression of placenta growth factor in proliferative diabetic retinopathy

Asud Khaliq, David Foreman, Asif Ahmed, Herbert Weich, Zdenek Gregor, David McLeod, Mike Boulton

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Proliferative diabetic retinopathy is thought to be mediated by the hypoxic regulation of angiogenic growth factors, in particular the vascular endothelial growth factor (VEGF) family. The aim of this study was to determine if placental growth factor (PIGF), a recently identified member of the VEGF family, was expressed in diabetic eyes undergoing preretinal neovascularization. Rabbit anti-PIGF antiserum was raised using a 20-amino acid N-terminal sequence to PIGF and did not cross react with VEGF185. Immunohistochemistry was performed on specimens of normal retina (n = 8), diabetic retina in the absence (n = 7) and presence (n = 4) of proliferative retinopathy, scatter laser-treated diabetic retina (n = 7), excised fibrovascular preretinal membranes (n = 12), and nondiabetic fibrocellular epiretinal (n = 7) membranes. PIGF levels were also determined in vitrectomy specimens from patients with either proliferative diabetic retinopathy or macular hole. PIGF immunoreactivity was intensely localized to the endothelial and perivascular regions of newly formed blood vessels of excised fibrovascular preretinal membranes. Intense localization of PIGF protein was also observed in superficial retinal vessels in diabetic retinae adjacent to neovascular preretinal membranes. Localization of PIGF was weak or absent in diabetic retinae that showed no evidence of neovascular proliferation. PIGF protein was also absent in normal retinae, in diabetic retinae that had received extensive treatment with scatter laser photocoagulation, and in nonvascularized epiretinal membranes. PIGF was present in all diabetic vitreous samples (mean 103 pg/ml) but nondetectable in control samples. These results strongly implicate a role for PIGF in the pathogenesis of proliferative diabetic retinopathy.

Original languageEnglish (US)
Pages (from-to)109-116
Number of pages8
JournalLaboratory Investigation
Volume78
Issue number1
StatePublished - 1998
Externally publishedYes

Fingerprint

Diabetic Retinopathy
Retina
Membranes
Vascular Endothelial Growth Factor A
Intercellular Signaling Peptides and Proteins
Lasers
Epiretinal Membrane
Retinal Vessels
Retinal Perforations
Light Coagulation
Angiogenesis Inducing Agents
Vitrectomy
Placenta Growth Factor
Blood Vessels
Immune Sera
Proteins
Immunohistochemistry
Rabbits
Amino Acids

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Khaliq, A., Foreman, D., Ahmed, A., Weich, H., Gregor, Z., McLeod, D., & Boulton, M. (1998). Increased expression of placenta growth factor in proliferative diabetic retinopathy. Laboratory Investigation, 78(1), 109-116.

Increased expression of placenta growth factor in proliferative diabetic retinopathy. / Khaliq, Asud; Foreman, David; Ahmed, Asif; Weich, Herbert; Gregor, Zdenek; McLeod, David; Boulton, Mike.

In: Laboratory Investigation, Vol. 78, No. 1, 1998, p. 109-116.

Research output: Contribution to journalArticle

Khaliq, A, Foreman, D, Ahmed, A, Weich, H, Gregor, Z, McLeod, D & Boulton, M 1998, 'Increased expression of placenta growth factor in proliferative diabetic retinopathy', Laboratory Investigation, vol. 78, no. 1, pp. 109-116.
Khaliq A, Foreman D, Ahmed A, Weich H, Gregor Z, McLeod D et al. Increased expression of placenta growth factor in proliferative diabetic retinopathy. Laboratory Investigation. 1998;78(1):109-116.
Khaliq, Asud ; Foreman, David ; Ahmed, Asif ; Weich, Herbert ; Gregor, Zdenek ; McLeod, David ; Boulton, Mike. / Increased expression of placenta growth factor in proliferative diabetic retinopathy. In: Laboratory Investigation. 1998 ; Vol. 78, No. 1. pp. 109-116.
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