Increased expression of transforming growth factor βs after acute oedematous pancreatitis in rats suggests a role in pancreatic repair

E. Riesle, H. Friess, L. Zhao, M. Wagner, W. Uhl, K. Baczako, L. I. Gold, Murray Korc, M. W. Büchler

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Background - Transforming growth factor β isoforms (TGFβs) belong to a family of multifunctional regulators of cellular growth and differentiation. They are mitogenic and chemotactic for fibroblasts and are potent stimulators of extracellular matrix production (collagen) and deposition. Upregulation of TGFβ transcription has been reported for several in vivo systems during repair after injury. Aims - To study the expression of the three mammalian isoforms of TGFβ (TGFβ1-3) and their relation to collagen expression as a marker for fibroblast response in acute oedematous pancreatitis in rats. Methods - Using northern blot analysis and immunohistochemistry, the expression and localisation of TGFβ isoforms, collagen, and amylase were analysed during the course of acute oedematous pancreatitis in rats, experimentally induced by intravenous caerulein infusion. Results - Induction of acute pancreatitis resulted in a biphasic peak pattern of expression of TGFβ1, β2, and β3 mRNA, with a pronounced increase from day 1 to day 3 (sixfold, 2.5-fold, fivefold, respectively) and again from day 5 to day 7 (threefold, 2.3-fold, 3.5-fold, respectively). The temporal changes in TGFβ mRNA identically paralleled the expression in collagen mRNA. In contrast, amylase mRNA expression, used as a general indicator of acinar cell integrity, was slightly decreased after induction of acute pancreatitis. Immunohistochemical analysis of pancreatitis tissue showed that increased expression of TGFβs was mainly present in the pancreatic acinar and ductal cells; this was evident within one day after pancreatitis induction. Conclusion - Overexpression of TGFβs after induction of acute pancreatitis suggests a role for these proteins in pancreatic repair and remodelling. The increased levels of TGFβs may help suppress immune activation, and may contribute to the increase in the extracellular matrix including collagen and to the repair of the pancreatic parenchyma.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalGut
Volume40
Issue number1
StatePublished - 1997
Externally publishedYes

Fingerprint

Transforming Growth Factors
Pancreatitis
Protein Isoforms
Collagen
Acinar Cells
Amylases
Messenger RNA
Extracellular Matrix
Fibroblasts
RNA Isoforms
Ceruletide
Intravenous Infusions
Northern Blotting
Up-Regulation
Immunohistochemistry
Wounds and Injuries
Growth

Keywords

  • Acute experimental pancreatitis
  • Amylase
  • Collagen
  • Pancreatic repair
  • Transforming growth factor β

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Riesle, E., Friess, H., Zhao, L., Wagner, M., Uhl, W., Baczako, K., ... Büchler, M. W. (1997). Increased expression of transforming growth factor βs after acute oedematous pancreatitis in rats suggests a role in pancreatic repair. Gut, 40(1), 73-79.

Increased expression of transforming growth factor βs after acute oedematous pancreatitis in rats suggests a role in pancreatic repair. / Riesle, E.; Friess, H.; Zhao, L.; Wagner, M.; Uhl, W.; Baczako, K.; Gold, L. I.; Korc, Murray; Büchler, M. W.

In: Gut, Vol. 40, No. 1, 1997, p. 73-79.

Research output: Contribution to journalArticle

Riesle, E, Friess, H, Zhao, L, Wagner, M, Uhl, W, Baczako, K, Gold, LI, Korc, M & Büchler, MW 1997, 'Increased expression of transforming growth factor βs after acute oedematous pancreatitis in rats suggests a role in pancreatic repair', Gut, vol. 40, no. 1, pp. 73-79.
Riesle, E. ; Friess, H. ; Zhao, L. ; Wagner, M. ; Uhl, W. ; Baczako, K. ; Gold, L. I. ; Korc, Murray ; Büchler, M. W. / Increased expression of transforming growth factor βs after acute oedematous pancreatitis in rats suggests a role in pancreatic repair. In: Gut. 1997 ; Vol. 40, No. 1. pp. 73-79.
@article{4d9aed0ea5594167b2a811828098d02e,
title = "Increased expression of transforming growth factor βs after acute oedematous pancreatitis in rats suggests a role in pancreatic repair",
abstract = "Background - Transforming growth factor β isoforms (TGFβs) belong to a family of multifunctional regulators of cellular growth and differentiation. They are mitogenic and chemotactic for fibroblasts and are potent stimulators of extracellular matrix production (collagen) and deposition. Upregulation of TGFβ transcription has been reported for several in vivo systems during repair after injury. Aims - To study the expression of the three mammalian isoforms of TGFβ (TGFβ1-3) and their relation to collagen expression as a marker for fibroblast response in acute oedematous pancreatitis in rats. Methods - Using northern blot analysis and immunohistochemistry, the expression and localisation of TGFβ isoforms, collagen, and amylase were analysed during the course of acute oedematous pancreatitis in rats, experimentally induced by intravenous caerulein infusion. Results - Induction of acute pancreatitis resulted in a biphasic peak pattern of expression of TGFβ1, β2, and β3 mRNA, with a pronounced increase from day 1 to day 3 (sixfold, 2.5-fold, fivefold, respectively) and again from day 5 to day 7 (threefold, 2.3-fold, 3.5-fold, respectively). The temporal changes in TGFβ mRNA identically paralleled the expression in collagen mRNA. In contrast, amylase mRNA expression, used as a general indicator of acinar cell integrity, was slightly decreased after induction of acute pancreatitis. Immunohistochemical analysis of pancreatitis tissue showed that increased expression of TGFβs was mainly present in the pancreatic acinar and ductal cells; this was evident within one day after pancreatitis induction. Conclusion - Overexpression of TGFβs after induction of acute pancreatitis suggests a role for these proteins in pancreatic repair and remodelling. The increased levels of TGFβs may help suppress immune activation, and may contribute to the increase in the extracellular matrix including collagen and to the repair of the pancreatic parenchyma.",
keywords = "Acute experimental pancreatitis, Amylase, Collagen, Pancreatic repair, Transforming growth factor β",
author = "E. Riesle and H. Friess and L. Zhao and M. Wagner and W. Uhl and K. Baczako and Gold, {L. I.} and Murray Korc and B{\"u}chler, {M. W.}",
year = "1997",
language = "English (US)",
volume = "40",
pages = "73--79",
journal = "Gut",
issn = "0017-5749",
publisher = "BMJ Publishing Group",
number = "1",

}

TY - JOUR

T1 - Increased expression of transforming growth factor βs after acute oedematous pancreatitis in rats suggests a role in pancreatic repair

AU - Riesle, E.

AU - Friess, H.

AU - Zhao, L.

AU - Wagner, M.

AU - Uhl, W.

AU - Baczako, K.

AU - Gold, L. I.

AU - Korc, Murray

AU - Büchler, M. W.

PY - 1997

Y1 - 1997

N2 - Background - Transforming growth factor β isoforms (TGFβs) belong to a family of multifunctional regulators of cellular growth and differentiation. They are mitogenic and chemotactic for fibroblasts and are potent stimulators of extracellular matrix production (collagen) and deposition. Upregulation of TGFβ transcription has been reported for several in vivo systems during repair after injury. Aims - To study the expression of the three mammalian isoforms of TGFβ (TGFβ1-3) and their relation to collagen expression as a marker for fibroblast response in acute oedematous pancreatitis in rats. Methods - Using northern blot analysis and immunohistochemistry, the expression and localisation of TGFβ isoforms, collagen, and amylase were analysed during the course of acute oedematous pancreatitis in rats, experimentally induced by intravenous caerulein infusion. Results - Induction of acute pancreatitis resulted in a biphasic peak pattern of expression of TGFβ1, β2, and β3 mRNA, with a pronounced increase from day 1 to day 3 (sixfold, 2.5-fold, fivefold, respectively) and again from day 5 to day 7 (threefold, 2.3-fold, 3.5-fold, respectively). The temporal changes in TGFβ mRNA identically paralleled the expression in collagen mRNA. In contrast, amylase mRNA expression, used as a general indicator of acinar cell integrity, was slightly decreased after induction of acute pancreatitis. Immunohistochemical analysis of pancreatitis tissue showed that increased expression of TGFβs was mainly present in the pancreatic acinar and ductal cells; this was evident within one day after pancreatitis induction. Conclusion - Overexpression of TGFβs after induction of acute pancreatitis suggests a role for these proteins in pancreatic repair and remodelling. The increased levels of TGFβs may help suppress immune activation, and may contribute to the increase in the extracellular matrix including collagen and to the repair of the pancreatic parenchyma.

AB - Background - Transforming growth factor β isoforms (TGFβs) belong to a family of multifunctional regulators of cellular growth and differentiation. They are mitogenic and chemotactic for fibroblasts and are potent stimulators of extracellular matrix production (collagen) and deposition. Upregulation of TGFβ transcription has been reported for several in vivo systems during repair after injury. Aims - To study the expression of the three mammalian isoforms of TGFβ (TGFβ1-3) and their relation to collagen expression as a marker for fibroblast response in acute oedematous pancreatitis in rats. Methods - Using northern blot analysis and immunohistochemistry, the expression and localisation of TGFβ isoforms, collagen, and amylase were analysed during the course of acute oedematous pancreatitis in rats, experimentally induced by intravenous caerulein infusion. Results - Induction of acute pancreatitis resulted in a biphasic peak pattern of expression of TGFβ1, β2, and β3 mRNA, with a pronounced increase from day 1 to day 3 (sixfold, 2.5-fold, fivefold, respectively) and again from day 5 to day 7 (threefold, 2.3-fold, 3.5-fold, respectively). The temporal changes in TGFβ mRNA identically paralleled the expression in collagen mRNA. In contrast, amylase mRNA expression, used as a general indicator of acinar cell integrity, was slightly decreased after induction of acute pancreatitis. Immunohistochemical analysis of pancreatitis tissue showed that increased expression of TGFβs was mainly present in the pancreatic acinar and ductal cells; this was evident within one day after pancreatitis induction. Conclusion - Overexpression of TGFβs after induction of acute pancreatitis suggests a role for these proteins in pancreatic repair and remodelling. The increased levels of TGFβs may help suppress immune activation, and may contribute to the increase in the extracellular matrix including collagen and to the repair of the pancreatic parenchyma.

KW - Acute experimental pancreatitis

KW - Amylase

KW - Collagen

KW - Pancreatic repair

KW - Transforming growth factor β

UR - http://www.scopus.com/inward/record.url?scp=0031043113&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031043113&partnerID=8YFLogxK

M3 - Article

C2 - 9155579

AN - SCOPUS:0031043113

VL - 40

SP - 73

EP - 79

JO - Gut

JF - Gut

SN - 0017-5749

IS - 1

ER -