Increased expression of type 2 cytokines in chronic proliferative dermatitis (cpdm) mutant mice and resolution of inflammation following treatment with IL-12

Harm HogenEsch, Sandra E. Torregrosa, Dawnalyn Boggess, Beth A. Sundberg, Joseph Carroll, John P. Sundberg

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Chronic proliferative dermatitis (cpdm) is a spontaneous mutation that results in eosinophilic inflammation in multiple tissues, including the skin. To determine the mechanisms underlying the eosinophilic inflammation, the expression of cytokines in the skin was determined. There was increased expression of IL-4, IL-5, IL-13, and granulocyte-macrophage colony-stimulating factor in the skin of cpdm/cpdm mice, and no change in IL-10 and TNF expression. Supernatants of cultured spleen cells of cpdm/cpdm mice contained an increased amount of IL-5 and IL-13, and a decreased amount of IFN-γ. The ability of the cpdm/cpdm mice to mount a delayed-type hypersensitivity response was greatly reduced. These data are consistent with impaired type 1 and excessive type 2 cytokine production in cpdm/cpdm mice. The significance of this imbalanced cytokine production was evident in the efficacy of systemic treatment of cpdm/cpdm mice with IL-12. Mutant mice treated for 3 weeks with IL-12 had minimal changes in the skin as opposed to the severe dermatitis in mice treated with the vehicle. Treatment with IL-11, which opposes the effect of IL-12, had no effect.

Original languageEnglish (US)
Pages (from-to)734-742
Number of pages9
JournalEuropean Journal of Immunology
Volume31
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Dermatitis
Interleukin-12
Cytokines
Inflammation
Skin
Interleukin-13
Interleukin-5
Interleukin-11
Delayed Hypersensitivity
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4
Interleukin-10
Cultured Cells
Spleen

Keywords

  • Cytokine
  • Eosinophil
  • IL-12
  • Mouse mutation
  • Skin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Increased expression of type 2 cytokines in chronic proliferative dermatitis (cpdm) mutant mice and resolution of inflammation following treatment with IL-12. / HogenEsch, Harm; Torregrosa, Sandra E.; Boggess, Dawnalyn; Sundberg, Beth A.; Carroll, Joseph; Sundberg, John P.

In: European Journal of Immunology, Vol. 31, No. 3, 2001, p. 734-742.

Research output: Contribution to journalArticle

HogenEsch, Harm ; Torregrosa, Sandra E. ; Boggess, Dawnalyn ; Sundberg, Beth A. ; Carroll, Joseph ; Sundberg, John P. / Increased expression of type 2 cytokines in chronic proliferative dermatitis (cpdm) mutant mice and resolution of inflammation following treatment with IL-12. In: European Journal of Immunology. 2001 ; Vol. 31, No. 3. pp. 734-742.
@article{d2f44450409443e5923db0195703b4ec,
title = "Increased expression of type 2 cytokines in chronic proliferative dermatitis (cpdm) mutant mice and resolution of inflammation following treatment with IL-12",
abstract = "Chronic proliferative dermatitis (cpdm) is a spontaneous mutation that results in eosinophilic inflammation in multiple tissues, including the skin. To determine the mechanisms underlying the eosinophilic inflammation, the expression of cytokines in the skin was determined. There was increased expression of IL-4, IL-5, IL-13, and granulocyte-macrophage colony-stimulating factor in the skin of cpdm/cpdm mice, and no change in IL-10 and TNF expression. Supernatants of cultured spleen cells of cpdm/cpdm mice contained an increased amount of IL-5 and IL-13, and a decreased amount of IFN-γ. The ability of the cpdm/cpdm mice to mount a delayed-type hypersensitivity response was greatly reduced. These data are consistent with impaired type 1 and excessive type 2 cytokine production in cpdm/cpdm mice. The significance of this imbalanced cytokine production was evident in the efficacy of systemic treatment of cpdm/cpdm mice with IL-12. Mutant mice treated for 3 weeks with IL-12 had minimal changes in the skin as opposed to the severe dermatitis in mice treated with the vehicle. Treatment with IL-11, which opposes the effect of IL-12, had no effect.",
keywords = "Cytokine, Eosinophil, IL-12, Mouse mutation, Skin",
author = "Harm HogenEsch and Torregrosa, {Sandra E.} and Dawnalyn Boggess and Sundberg, {Beth A.} and Joseph Carroll and Sundberg, {John P.}",
year = "2001",
doi = "10.1002/1521-4141(200103)31:3<734::AID-IMMU734>3.0.CO;2-9",
language = "English (US)",
volume = "31",
pages = "734--742",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "3",

}

TY - JOUR

T1 - Increased expression of type 2 cytokines in chronic proliferative dermatitis (cpdm) mutant mice and resolution of inflammation following treatment with IL-12

AU - HogenEsch, Harm

AU - Torregrosa, Sandra E.

AU - Boggess, Dawnalyn

AU - Sundberg, Beth A.

AU - Carroll, Joseph

AU - Sundberg, John P.

PY - 2001

Y1 - 2001

N2 - Chronic proliferative dermatitis (cpdm) is a spontaneous mutation that results in eosinophilic inflammation in multiple tissues, including the skin. To determine the mechanisms underlying the eosinophilic inflammation, the expression of cytokines in the skin was determined. There was increased expression of IL-4, IL-5, IL-13, and granulocyte-macrophage colony-stimulating factor in the skin of cpdm/cpdm mice, and no change in IL-10 and TNF expression. Supernatants of cultured spleen cells of cpdm/cpdm mice contained an increased amount of IL-5 and IL-13, and a decreased amount of IFN-γ. The ability of the cpdm/cpdm mice to mount a delayed-type hypersensitivity response was greatly reduced. These data are consistent with impaired type 1 and excessive type 2 cytokine production in cpdm/cpdm mice. The significance of this imbalanced cytokine production was evident in the efficacy of systemic treatment of cpdm/cpdm mice with IL-12. Mutant mice treated for 3 weeks with IL-12 had minimal changes in the skin as opposed to the severe dermatitis in mice treated with the vehicle. Treatment with IL-11, which opposes the effect of IL-12, had no effect.

AB - Chronic proliferative dermatitis (cpdm) is a spontaneous mutation that results in eosinophilic inflammation in multiple tissues, including the skin. To determine the mechanisms underlying the eosinophilic inflammation, the expression of cytokines in the skin was determined. There was increased expression of IL-4, IL-5, IL-13, and granulocyte-macrophage colony-stimulating factor in the skin of cpdm/cpdm mice, and no change in IL-10 and TNF expression. Supernatants of cultured spleen cells of cpdm/cpdm mice contained an increased amount of IL-5 and IL-13, and a decreased amount of IFN-γ. The ability of the cpdm/cpdm mice to mount a delayed-type hypersensitivity response was greatly reduced. These data are consistent with impaired type 1 and excessive type 2 cytokine production in cpdm/cpdm mice. The significance of this imbalanced cytokine production was evident in the efficacy of systemic treatment of cpdm/cpdm mice with IL-12. Mutant mice treated for 3 weeks with IL-12 had minimal changes in the skin as opposed to the severe dermatitis in mice treated with the vehicle. Treatment with IL-11, which opposes the effect of IL-12, had no effect.

KW - Cytokine

KW - Eosinophil

KW - IL-12

KW - Mouse mutation

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=0035081842&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035081842&partnerID=8YFLogxK

U2 - 10.1002/1521-4141(200103)31:3<734::AID-IMMU734>3.0.CO;2-9

DO - 10.1002/1521-4141(200103)31:3<734::AID-IMMU734>3.0.CO;2-9

M3 - Article

C2 - 11241277

AN - SCOPUS:0035081842

VL - 31

SP - 734

EP - 742

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 3

ER -