Increased glycogen storage in yeast results in less branched glycogen

Wayne A. Wilson, William E. Hughes, Wendy Tomamichel, Peter Roach

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Glycogen is a branched polymer of glucose, synthesized as a reserve of both energy and carbon. The branched nature of glycogen is important for its function and polyglucosan bodies, particles that contain a glycogen-like polymer with reduced branching, are a feature of several disease states. The degree of glycogen branching is thought to be governed by the balance between glycogen synthesis and branching activities. However, there have been reports that the intrinsic properties of individual branching enzymes govern the degree of branching. To address the relationship between synthesis and branching more fully, we made use of the yeast Saccharomyces cerevisiae. The glycogen content of yeast cells was manipulated by using different growth conditions or by the introduction of specific mutations. Whenever glycogen storage was elevated, the polysaccharide formed was found to be less branched but normal branching could be restored by overexpression of branching enzyme.

Original languageEnglish
Pages (from-to)416-423
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume320
Issue number2
DOIs
StatePublished - Jul 23 2004

Fingerprint

Glycogen
Yeast
Yeasts
1,4-alpha-Glucan Branching Enzyme
Glucans
Polysaccharides
Saccharomyces cerevisiae
Polymers
Carbon
Cells
Mutation
Growth

Keywords

  • Branching
  • Glycogen
  • Polyglucosan
  • Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Increased glycogen storage in yeast results in less branched glycogen. / Wilson, Wayne A.; Hughes, William E.; Tomamichel, Wendy; Roach, Peter.

In: Biochemical and Biophysical Research Communications, Vol. 320, No. 2, 23.07.2004, p. 416-423.

Research output: Contribution to journalArticle

Wilson, Wayne A. ; Hughes, William E. ; Tomamichel, Wendy ; Roach, Peter. / Increased glycogen storage in yeast results in less branched glycogen. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 320, No. 2. pp. 416-423.
@article{d0058840dd10452186a16f62ddecfcab,
title = "Increased glycogen storage in yeast results in less branched glycogen",
abstract = "Glycogen is a branched polymer of glucose, synthesized as a reserve of both energy and carbon. The branched nature of glycogen is important for its function and polyglucosan bodies, particles that contain a glycogen-like polymer with reduced branching, are a feature of several disease states. The degree of glycogen branching is thought to be governed by the balance between glycogen synthesis and branching activities. However, there have been reports that the intrinsic properties of individual branching enzymes govern the degree of branching. To address the relationship between synthesis and branching more fully, we made use of the yeast Saccharomyces cerevisiae. The glycogen content of yeast cells was manipulated by using different growth conditions or by the introduction of specific mutations. Whenever glycogen storage was elevated, the polysaccharide formed was found to be less branched but normal branching could be restored by overexpression of branching enzyme.",
keywords = "Branching, Glycogen, Polyglucosan, Yeast",
author = "Wilson, {Wayne A.} and Hughes, {William E.} and Wendy Tomamichel and Peter Roach",
year = "2004",
month = "7",
day = "23",
doi = "10.1016/j.bbrc.2004.05.180",
language = "English",
volume = "320",
pages = "416--423",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Increased glycogen storage in yeast results in less branched glycogen

AU - Wilson, Wayne A.

AU - Hughes, William E.

AU - Tomamichel, Wendy

AU - Roach, Peter

PY - 2004/7/23

Y1 - 2004/7/23

N2 - Glycogen is a branched polymer of glucose, synthesized as a reserve of both energy and carbon. The branched nature of glycogen is important for its function and polyglucosan bodies, particles that contain a glycogen-like polymer with reduced branching, are a feature of several disease states. The degree of glycogen branching is thought to be governed by the balance between glycogen synthesis and branching activities. However, there have been reports that the intrinsic properties of individual branching enzymes govern the degree of branching. To address the relationship between synthesis and branching more fully, we made use of the yeast Saccharomyces cerevisiae. The glycogen content of yeast cells was manipulated by using different growth conditions or by the introduction of specific mutations. Whenever glycogen storage was elevated, the polysaccharide formed was found to be less branched but normal branching could be restored by overexpression of branching enzyme.

AB - Glycogen is a branched polymer of glucose, synthesized as a reserve of both energy and carbon. The branched nature of glycogen is important for its function and polyglucosan bodies, particles that contain a glycogen-like polymer with reduced branching, are a feature of several disease states. The degree of glycogen branching is thought to be governed by the balance between glycogen synthesis and branching activities. However, there have been reports that the intrinsic properties of individual branching enzymes govern the degree of branching. To address the relationship between synthesis and branching more fully, we made use of the yeast Saccharomyces cerevisiae. The glycogen content of yeast cells was manipulated by using different growth conditions or by the introduction of specific mutations. Whenever glycogen storage was elevated, the polysaccharide formed was found to be less branched but normal branching could be restored by overexpression of branching enzyme.

KW - Branching

KW - Glycogen

KW - Polyglucosan

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=2942715072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942715072&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2004.05.180

DO - 10.1016/j.bbrc.2004.05.180

M3 - Article

C2 - 15219844

AN - SCOPUS:2942715072

VL - 320

SP - 416

EP - 423

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -