Increased mandibular condylar growth in mice with estrogen receptor beta deficiency

Yosuke Kamiya, Jing Chen, Manshan Xu, Achint Utreja, Thomas Choi, Hicham Drissi, Sunil Wadhwa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Temporomandibular joint (TMJ) disorders predominantly afflict women of childbearing age, suggesting a role for female hormones in the disease process. In long bones, estrogen acting via estrogen receptor beta (ERβ) inhibits axial skeletal growth in female mice. However, the role of ERβ in the mandibular condyle is largely unknown. We hypothesize that female ERβ-deficient mice will have increased mandibular condylar growth compared to wild-type (WT) female mice. This study examined female 7-day-old, 49-day-old, and 120-day-old WT and ERβ knockout (KO) mice. There was a significant increase in mandibular condylar cartilage thickness as a result of an increased number of cells, in the 49-day-old and 120-day-old female ERβ KO compared with WT controls. Analysis in 49-day-old female ERβ KO mice revealed a significant increase in collagen type X, parathyroid hormone-related protein (Pthrp), and osteoprotegerin gene expression and a significant decrease in receptor activator for nuclear factor κ B ligand (Rankl) and Indian hedgehog (Ihh) gene expression, compared with WT controls. Subchondral bone analysis revealed a significant increase in total condylar volume and a decrease in the number of osteoclasts in the 49-day-old ERβ KO compared with WT female mice. There was no difference in cell proliferation in condylar cartilage between the genotypes. However, there were differences in the expression of proteins that regulate the cell cycle; we found a decrease in the expression of Tieg1 and p57 in the mandibular condylar cartilage from ERβ KO mice compared with WT mice. Taken together, our results suggest that ERβ deficiency increases condylar growth in female mice by inhibiting the turnover of fibrocartilage. © 2013 American Society for Bone and Mineral Research.

Original languageEnglish (US)
Pages (from-to)1127-1134
Number of pages8
JournalJournal of Bone and Mineral Research
Volume28
Issue number5
DOIs
StatePublished - May 1 2013

Fingerprint

Estrogen Receptor beta
Growth
Knockout Mice
Cartilage
Collagen Type X
Fibrocartilage
Mandibular Condyle
Gene Expression
Parathyroid Hormone-Related Protein
Osteoprotegerin
Bone and Bones
Temporomandibular Joint Disorders
Cell Cycle Proteins
Hedgehogs
Osteoclasts
Cytoplasmic and Nuclear Receptors
Estrogens
Cell Count
Genotype
Cell Proliferation

Keywords

  • Bone Histomorphometry
  • Chondrocyte, Epiphysis And Cartilage Biology
  • Estrogens And Serms
  • Joint
  • Rodent

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

Cite this

Kamiya, Y., Chen, J., Xu, M., Utreja, A., Choi, T., Drissi, H., & Wadhwa, S. (2013). Increased mandibular condylar growth in mice with estrogen receptor beta deficiency. Journal of Bone and Mineral Research, 28(5), 1127-1134. https://doi.org/10.1002/jbmr.1835

Increased mandibular condylar growth in mice with estrogen receptor beta deficiency. / Kamiya, Yosuke; Chen, Jing; Xu, Manshan; Utreja, Achint; Choi, Thomas; Drissi, Hicham; Wadhwa, Sunil.

In: Journal of Bone and Mineral Research, Vol. 28, No. 5, 01.05.2013, p. 1127-1134.

Research output: Contribution to journalArticle

Kamiya, Y, Chen, J, Xu, M, Utreja, A, Choi, T, Drissi, H & Wadhwa, S 2013, 'Increased mandibular condylar growth in mice with estrogen receptor beta deficiency', Journal of Bone and Mineral Research, vol. 28, no. 5, pp. 1127-1134. https://doi.org/10.1002/jbmr.1835
Kamiya, Yosuke ; Chen, Jing ; Xu, Manshan ; Utreja, Achint ; Choi, Thomas ; Drissi, Hicham ; Wadhwa, Sunil. / Increased mandibular condylar growth in mice with estrogen receptor beta deficiency. In: Journal of Bone and Mineral Research. 2013 ; Vol. 28, No. 5. pp. 1127-1134.
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