Increased MAPK expression and activity in primary human hepatocellular carcinoma

C. Schmidt, Iain H. McKillop, Paul A. Cahill, James V. Sitzmann

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

We investigated the expression and activity of mitogen-activated protein kinase (MAPK) in human hepatocellular carcinoma (HCC). MAPK expression was determined in five human tumors and five normal tissues (adjacent non-neoplastic liver) by Western blotting using specific antisera raised against four MAPK pathway intermediates: Erk-l, Erk-2 (extracellular-signal regulated kinases), Mek-l and Mek-2 (mitogen activated protein kinase kinases). There was a significant increase in Erk-1, Erk-2, Mek-1 and Mek-2 expression in particulate and cytosolic fractions prepared from tumor specimens as compared with the adjacent normal control tissues. The functional activity of both membrane and cytosolic Erk-2, determined by phosphorylation of myelin basic protein (MBP), was significantly increased in tumor specimens as compared to normal (membrane: 321% ± 50%, p <0.05; and cytosol: 597% ± 233%, p <0.05 percent of normal tissue). These data demonstrate for the first time a significant increase in MAPK expression and functional activity in human HCC. Because of the important role that the MAPK pathway plays in cellular growth and differentiation, overexpression of MAPK may be of critical importance to the formation and maintenance of human hepatocellular carcinoma.

Original languageEnglish (US)
Pages (from-to)54-58
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume236
Issue number1
DOIs
StatePublished - Jul 9 1997
Externally publishedYes

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Mitogen-Activated Protein Kinases
Hepatocellular Carcinoma
Tumors
Tissue
MAP Kinase Kinase 2
Membranes
Neoplasms
Phosphorylation
Myelin Basic Protein
Mitogen-Activated Protein Kinase 1
Human Activities
Liver
Cytosol
Immune Sera
Western Blotting
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Increased MAPK expression and activity in primary human hepatocellular carcinoma. / Schmidt, C.; McKillop, Iain H.; Cahill, Paul A.; Sitzmann, James V.

In: Biochemical and Biophysical Research Communications, Vol. 236, No. 1, 09.07.1997, p. 54-58.

Research output: Contribution to journalArticle

Schmidt, C. ; McKillop, Iain H. ; Cahill, Paul A. ; Sitzmann, James V. / Increased MAPK expression and activity in primary human hepatocellular carcinoma. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 236, No. 1. pp. 54-58.
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