Increased ouabain‐sensitive glycolysis of lymphocytes treated with phytohemagglutinin: Relationship to potassium transport

George B. Segel, Elliot J. Androphy, Marshall A. Lichtman

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

An early increase in lymphocyte plasma membrane K+ transport is essential for PHA stimulated lymphocytes to divide. Little is known about the specific source and amount of energy required to support the increased transport by activated lymphocytes. Since ouabain, a cardiac glycoside, specifically inhibits the transport ATPase, we have measured the decrement in glycolysis and tricarboxylic acid cycle activity when untreated and PHA treated lymphocytes were exposed to ouabain. This metabolic decrement represents the portion of metabolism associated with monovalent cation transport and closely related processes. Since TCA cycle activity accounted for only 0.2% of glucose consumption, aerobic glycolysis was the major source of energy, i.e., ATP, for increased transport. Approximately one‐third of the total lactate production in both control and PHA stimulated lymphocytes was ouabain‐sensitive. Ouabain sensitive lactate production in control, 105 μmol/1010 cells/hour, increased 1.8‐fold to 193 μmol/1010 cells/hour after PHA treatment. Active K+ influx in similar cell populations increased from 40 μmol/1010 cells/hour to 74 μmol/1010 cells/hour (1.9‐fold) after PHA treatment. The increment in ouabain‐sensitive energy production and K+ transport were closely correlated and, therefore, 0.38 moles of K+ are transported for each mole of ATP generated in both control and PHA treated cells. The increased requirement for transport related energy is provided by increasing the ouabain‐sensitive ATP production rather than altering the efficiency of ATP transduction.

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalJournal of cellular physiology
Volume97
Issue number3
DOIs
StatePublished - Dec 1978
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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