Increased strontium uptake in trabecular bone of ovariectomized calcium-deficient rats treated with strontium ranelate or strontium chloride

Bernhard Pemmer, Jochen G. Hofstaetter, Florian Meirer, Stephan Smolek, Peter Wobrauschek, Rolf Simon, Robyn K. Fuchs, Matthew Allen, Keith W. Condon, Susan Reinwald, Roger J. Phipps, David Burr, Eleftherios P. Paschalis, Klaus Klaushofer, Christina Streli, Paul Roschger

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Based on clinical trials showing the efficacy to reduce vertebral and non-vertebral fractures, strontium ranelate (SrR) has been approved in several countries for the treatment of postmenopausal osteoporosis. Hence, it is of special clinical interest to elucidate how the Sr uptake is influenced by dietary Ca deficiency as well as by the formula of Sr administration, SrR versus strontium chloride (SrCl2). Three-month-old ovariectomized rats were treated for 90 days with doses of 25 mg kg-1 d-1 and 150 mg kg-1 d-1 of SrR or SrCl2 at low (0.1% Ca) or normal (1.19% Ca) Ca diet. Vertebral bone tissue was analysed by confocal synchrotron-radiation-induced micro X-ray fluorescence and by backscattered electron imaging. Principal component analysis and k-means clustering of the acquired elemental maps of Ca and Sr revealed that the newly formed bone exhibited the highest Sr fractions and that low Ca diet increased the Sr uptake by a factor of three to four. Furthermore, Sr uptake in bone of the SrCl 2-treated animals was generally lower compared with SrR. The study clearly shows that inadequate nutritional calcium intake significantly increases uptake of Sr in serum as well as in trabecular bone matrix. This indicates that nutritional calcium intake as well as serum Ca levels are important regulators of any Sr treatment.

Original languageEnglish
Pages (from-to)835-841
Number of pages7
JournalJournal of Synchrotron Radiation
Volume18
Issue number6
DOIs
StatePublished - Nov 2011

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Strontium
strontium
bones
rats
Rats
calcium
Calcium
Bone
chlorides
diets
Nutrition
serums
osteoporosis
regulators
principal components analysis
Synchrotron radiation
Principal component analysis
animals
synchrotron radiation
Animals

Keywords

  • dietary calcium
  • elemental mapping
  • k-means clustering
  • ovariectomized rats
  • principal component analysis
  • qBEI
  • SR μ-XRF
  • strontium chloride
  • strontium ranelate

ASJC Scopus subject areas

  • Instrumentation
  • Nuclear and High Energy Physics
  • Radiation

Cite this

Increased strontium uptake in trabecular bone of ovariectomized calcium-deficient rats treated with strontium ranelate or strontium chloride. / Pemmer, Bernhard; Hofstaetter, Jochen G.; Meirer, Florian; Smolek, Stephan; Wobrauschek, Peter; Simon, Rolf; Fuchs, Robyn K.; Allen, Matthew; Condon, Keith W.; Reinwald, Susan; Phipps, Roger J.; Burr, David; Paschalis, Eleftherios P.; Klaushofer, Klaus; Streli, Christina; Roschger, Paul.

In: Journal of Synchrotron Radiation, Vol. 18, No. 6, 11.2011, p. 835-841.

Research output: Contribution to journalArticle

Pemmer, B, Hofstaetter, JG, Meirer, F, Smolek, S, Wobrauschek, P, Simon, R, Fuchs, RK, Allen, M, Condon, KW, Reinwald, S, Phipps, RJ, Burr, D, Paschalis, EP, Klaushofer, K, Streli, C & Roschger, P 2011, 'Increased strontium uptake in trabecular bone of ovariectomized calcium-deficient rats treated with strontium ranelate or strontium chloride', Journal of Synchrotron Radiation, vol. 18, no. 6, pp. 835-841. https://doi.org/10.1107/S090904951103038X
Pemmer, Bernhard ; Hofstaetter, Jochen G. ; Meirer, Florian ; Smolek, Stephan ; Wobrauschek, Peter ; Simon, Rolf ; Fuchs, Robyn K. ; Allen, Matthew ; Condon, Keith W. ; Reinwald, Susan ; Phipps, Roger J. ; Burr, David ; Paschalis, Eleftherios P. ; Klaushofer, Klaus ; Streli, Christina ; Roschger, Paul. / Increased strontium uptake in trabecular bone of ovariectomized calcium-deficient rats treated with strontium ranelate or strontium chloride. In: Journal of Synchrotron Radiation. 2011 ; Vol. 18, No. 6. pp. 835-841.
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abstract = "Based on clinical trials showing the efficacy to reduce vertebral and non-vertebral fractures, strontium ranelate (SrR) has been approved in several countries for the treatment of postmenopausal osteoporosis. Hence, it is of special clinical interest to elucidate how the Sr uptake is influenced by dietary Ca deficiency as well as by the formula of Sr administration, SrR versus strontium chloride (SrCl2). Three-month-old ovariectomized rats were treated for 90 days with doses of 25 mg kg-1 d-1 and 150 mg kg-1 d-1 of SrR or SrCl2 at low (0.1{\%} Ca) or normal (1.19{\%} Ca) Ca diet. Vertebral bone tissue was analysed by confocal synchrotron-radiation-induced micro X-ray fluorescence and by backscattered electron imaging. Principal component analysis and k-means clustering of the acquired elemental maps of Ca and Sr revealed that the newly formed bone exhibited the highest Sr fractions and that low Ca diet increased the Sr uptake by a factor of three to four. Furthermore, Sr uptake in bone of the SrCl 2-treated animals was generally lower compared with SrR. The study clearly shows that inadequate nutritional calcium intake significantly increases uptake of Sr in serum as well as in trabecular bone matrix. This indicates that nutritional calcium intake as well as serum Ca levels are important regulators of any Sr treatment.",
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AU - Smolek, Stephan

AU - Wobrauschek, Peter

AU - Simon, Rolf

AU - Fuchs, Robyn K.

AU - Allen, Matthew

AU - Condon, Keith W.

AU - Reinwald, Susan

AU - Phipps, Roger J.

AU - Burr, David

AU - Paschalis, Eleftherios P.

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AU - Streli, Christina

AU - Roschger, Paul

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